House dust test procedure or other building dust sampling for fiberglass:
This indoor air quality testing article describes the process for laboratory identification of particles found in indoor air or settled dust collected in residential and light-commercial buildings.
Typical components of house dust are dominated by fabric fibers and skin cells. We may however find either high levels of common problem particles (mold, allergens, fiberglass or other insulation fragments) or low levels of particles that by their nature still indicate a problem.
Here we include photographs of unbonded fiberglass insulation - blowing wools. How to distinguish between fiberglass fibers & fabric fibers in house dust. Microscope procedures for identification of fiberglass. Fiberglass lab test warnings & tips.
Our page top photo shows a vacuum sample of fiberglass building insulation. The bonding resin is plainly visible in our lab photo - often the color of the binding resin in fiberglass insulation helps trace insulation dust in a building back to its source. Not all fiberglass insulation includes resin binders however.
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This article describes easy steps taken in the forensic lab that permit reliable particle identification and distinguishing among fiberglass insulation, mineral wool or "rock wool" and cotton or other fibers.
Above we show a macro photograph of white blown-in unbonded InsulSafe® building insulation sold by CertainTeed and provided by a homeowner who asked our lab to study dust samples from her home.
At above is the same insulation shown in the stereo microscope at about 20x, and below the same material is magnified to 720x.
Above we show a 720x micro-photograph of white blown-in unbonded InsulSafe® building insulation sold by CertainTeed.
Above our photo shows the dominant particles in the dust sample from the home under study.
Magnified to 720x the fibers we found were primarily cotton, with some linen and a few synthetic fabric fibers.
There was virtually none of the insulation fibers provided for comparison (above left) as a possible source of dust in the home.
Above a client photo shows a heavy and rapid dust accumulation on building surfaces.
Above our lab photo shows that the prime contents of the dust were fabric fibers and starch granules, not building insulation in this case. - DF & WW 6/2010.
Watch out: when preparing a laboratory sample for microscopic examination for the present and level of fiberglass fragments (from insulation or any other source), choose the mountant liquid wisely.
If you use a slide mountant whose refractive index is close to that of glass fibers, detection of small fiberglass fragments may be difficult or impossible even though they may be present at high levels.
Thus while it may be trivial to distinguish fiberglass from other fibers found in building dust, it the mountant is not properly selected (with the proper n or refractive index), or if the microscope is not properly adjusted, the technician can completely miss high levels of ultra-fine fiberglass fragments.
While detection of large fibers of any sort in a microscope sample is relatively easy, in our OPINION, some fiberglass dust studies have been faulty in design because technicians seriously under-report the presence of ultra-small fiberglass insulation fragments in dust samples.
This error occurs because of a combination of:
In sum, as forensic microscopists know well, if you don't look for a particle or a particle in the proper size range, preparation, and magnification, you will not find it.
Details about the problems of detecting ultra-small fiberglass fragments are
at FIBERGLASS DETECTION in BUILDING AIR & DUST
...
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