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Water Tests for Swimming Safety
Water test options & standards for swimming safety at lakes & at salt water beaches
POST a QUESTION or COMMENT about how to test beach water, pond water, swimming water, or similar water bodies for safety and for contaminants
Water safety testing for coliform levels: water test options, procedures & standards for checking the safety of water at swimming areas for lakes, ponds, streams, rivers, and ocean beaches.
Water test options & recommendations for fresh water & salt water beaches, also ponds and swimming holes, lakes, and streams.
This article describes useful methods for testing swimming beach water for safety at fresh water and salt water beaches.
We discuss the common water contamination concerns, the telltale or most common markers or tests such as testing for coliform or E-coli bacteria in swimming water, and we'll discuss special measures appropriate when testing for coliform in salt water.
The article includes research citations, water testing standards citations, and sources for water testing materials.
Page top photo: children learning to swim at Manzanilla in southern Costa Rica.
InspectAPedia tolerates no conflicts of interest. We have no relationship with advertisers, products, or services discussed at this website.
- Daniel Friedman, Publisher/Editor/Author - See WHO ARE WE?
Ocean & Fresh Water Beach Swimming Water Safety & Sanitation Tests
Our associate Steve Vermilye (d. 2001)
worked as a teenager rowing a River Keeper about in New York's lower Hudson River where they spotted and reported industrial and sewage pipes spilling directly into the river.
[Click to enlarge any image]
Some similar contaminant sources continue around the world today. However according to the U.S. NRDC every U.S. coastal state has at least one beach with pollution problems. (NRDC 2014)
While most sources blame storm water for beach pollution (heavy rains overwhelm local sewage processing plants causing discharge of sewage into the ocean), there may be other pollution sources that have not been detected.
Watch out: when considering swimming beach safety, don't worry so much about water pollution that you fail to observe other serious and immediate hazards such as riptides, lightning hazards, cuts from stepping on debris, or alligators such as this one waiting patiently for us to step into her water at an estuary spilling into the ocean after a storm at La Manzanilla on the west coast of Mexico.
Storm waters wash not only contaminants but also alligators out into the ocean. Preferring inland waters these alligators make their way back upstream following the storm.
Water test kit providers for swimming water, lake water, ocean water testing for sewage contaminants - Marine Water Testing Kits & Procedures
Ammonia test kits for sea water: Hanna Instruments provides the HI 3826 Ammonia Test Kit for Sea Water, a colorimetric test for ammonia in seawater, typically operating in the 0.0 - 2.5 mg/L range using the Nessler chemical method are sold for measuring the concentration of ammonia in rivers and drinking water reservoirs.
Ammonia in turn may be an indicator of other types of contaminants. This is not a direct test for pathogens such as Total coliform or E. coli.
elAgua Water Test Kits, DelAgua Water Testing Limited
Unit 2
The Old Dairy
Church Lane
Lower Fyfield
Marlborough
SN8 1PX
United Kingdom, Tel: +44-0-1672-861-198, Email: info@delagua.org [Pending company response - Ed.]
Enterolert by IDEXX, distributing water test materials world wide, IDEXX Laboratories, Inc.
One IDEXX Drive
Westbrook, Maine 04092 USA
Tel: 1-207-556-4496 or
Tel: 1-800-321-0207
Fax: 1-207-556-4630
, Email: water@idexx.com.
Website: https://www.idexx.com
The Enteroalert® test is a 24-hour test for the detection of Enterococci using ASTM MEthod #D6503-99. This test for enterococci in water
is the "gold standard" for testing beaches. The tests are simple and inexpensive; unfortunately the lab needs a $4000 machine plus an incubator, UV light and some other gear in order to process the test samples - equipment that most small water testing labs around the world simply don't have installed and probably cannot afford.
Quoting:
Less than 1 minute of hands-on time.
Results in 24 hours rather than 48 to 72 hours.
Sensitive to 1 enterococci/100 mL.
Enumerates up to 2,419 enterococci per 100 mL without dilutions (with Quanti-Tray®/2000).
Less subjective interpretation.
50% fewer false positives and 95% fewer false negatives than the standard membrane filtration (MF) method.1
Up to 12-month shelf life minimizes waste.
24-hour test saves incubator space.
Parker Kittiwake, Marine Water & Sewage Effluent TEst Kits, Website: http://www.kittiwake.com/marine-water-test-kits-systems, Tel: USA: 1-713-255-7255, UK +44 1 903 731 470 The company provides other marine test kits as well, such as a Marine Hygiene Test Kit for Legionella bacteria used at offshore installations. Quoting the company's description of their Marine Water test kit:
This is a legislation driven requirement under the ILO Maritime Labour Convention 2006. Suitable control schemes with adequate marine water testing and monitoring will aid with the compliance of the current IHR (2005), ILO 178 (2009) and ILO MLC (2006), which enters into force from January 2012.
These products provide real time analysis and simple to perform tests needing no specialist training.
Included among the company's test are : Biological - Coliform, E.Coli, Pseudomonas Aeruginosa, Enterococci, HPC (Total viable count).
Simpltek Water Testing,
Simpltek, 8 Byron Road. Commack, NY 11725, Tel: 1-631-864-0099,
Email: info@simpltek.com, or sales@simpltek.com, Website: http://www.simpltek.com.
Alex Astachovsky at Simpltek offered this advice for beach water sanitation safety testing:
We recommend a professional Micro Tester Pro box 30 or 8 tests for multiple testing. If the user needs to do a few tests, they can use single stand alone Micro Inspector consumer test.
We also offering a variety of chemical tests: Ammonia Nitrogen, Chlorine DPD, Hardness, Iron, Phosphate Ortho and Zinc in professional and consumer format.
Coliform testing in fresh water or marine waters: the company informs us that the following two coliform test kits will perform adequately in marine waters (seawater or ocean beach water). Product instructions are given here:
MICRO INSPECTORColiform [PDF] a consumer water test kit using a self-filling ampoule & syringe.
MICRO TESTER PRO Pro Coliform [PDF] Professional water test kit for users needing to perform multiple tests.
The Coliform test will perform "as is" (no dilution or other requirements) in fresh or sea water.
Simpltek also suggests these other biological tests:
E-colitest, no specific requirements for fresh water testing. To eliminate false positive results for marine water dilution 1:10 - 1:100 required. - Needed: a Black (UV) Light to view a test results (BLACK LIGHT & UV LIGHT USES).
MICRO TESTER for E-Coli [PDF] a consumer water test kit using a self-filling ampoule & syringe.
MICRO TESTER PRO E-Coli [PDF] a professional water test kit for users needing to perform multiple tests
Total Microbe test, from Simpltek will detect any/all aerobic and facultative bacteria, non specifically (Coliform, E-coli, Giardia, etc, it's a huge list). Simpltek points out that this test is very important for this simple reason - when you are testing for specific bacteria, you missing others. It will perform "as is" (no dilution or other requirements) in fresh or sea water.
However as we explain
at WATER TEST PROCEDURE, testing for Coliform or E-Coli is recommended by the US EPA and other expert sources as a most useful general screen for biological water contaminants - Ed.
Watch out: testing recommendations for swimming water safety vary between fresh water and salt water. At "Clean Beach Water Testing Guides" in this article (below) see our US EPA excerpts pointing out the importance of testing marine waters (salt water) for enterococci rather than relying on E-coli tests (which are fine for fresh water).
Watch out: also that testing methodology is as important as using a recommended test method for swimming or recreational water sanitation- safety. For example the frequency and response of water sample collection after events (e.g. storms) will be as critical as using a recommended water testing method.
In short, exactly how, where, and how often water samples are collected are important in forming reliable beach safety or sanitation test data.
Watch outfinally for confusing precision (precise counts of E-coli or Enterococci) versus accuracy. Water sampling in large bodies of water is vulnerable to more variation among individual samples than you might guess, even when samples are collected at the same time and very nearly at the same location. - Boehm (2003) & Santoro, Boehm et als (2008).
And Wheeler et als (2003) report that these bacteria may be present even in wet beach sand (you don't have to go into the water) while others report the bacteria may be reintroduced into water even from temporary residence in dry beach sand - Abdelzaher (2010).
Slanetz & Bartley MF Media is a dehydrated agar-based culture media (recipe includes about 8 ingredients) used to test for enterococci. This test was originally intended for water supplies (that is, not swimming beaches) and is described as follows, quoting from Oxoid,
Technique: The Environment Agency ’Microbiology of Drinking Water 2002’6 recommend the use of Slanetz and Bartley medium for the enumeration of enterococci in water supplies, as do ISO in the standard for water quality7. The water is filtered through a membrane filter which is then placed on the surface of a well dried plate of the medium.
Plates are incubated at 35°C for 4 hours and then at 44-45°C for 44 hours. Membranes are examined, with a hand lens in a good light, and all red or maroon colonies counted as enterococci.
... Slanetz & Bartley1 originally devised this medium to detect and enumerate enterococci by the technique of membrane filtration, but it has also proved useful as a direct plating medium2,3.
The medium is very selective for enterococci and, when it is incubated at elevated temperatures (44-45°C), all red or maroon colonies may be accepted as presumptive enterococci4,5.
Burkwall and Hartman showed that the addition of 0.5ml of `Tween 80’ and 20ml of a 10% solution of sodium carbonate or bicarbonate to each litre of medium of a modified formulation of Slanetz and Bartley Medium was of value when examining frozen foods for enterococci;
the original article (Burkwall M. K. and Hartman P. A. (1964) Appl. Microbiol. 12. 18-23.) should be consulted for procedural details.
Slanetz & Bartley MF Media and Quanti-Tray® have been studied (by IDEXX) and compared to these methods for testing for Enterococci in recreational waters. IDEXX reports that both methods produced comparable results and that Enteroalert had a 25% lower false positive result rate (4.2% vs 6.2% in absolute numbers) than the Slanetz & Bartley Media approach. - retrieved 8/22/14, original source: http://www.oxoid.com/uk/blue/prod_detail/prod_detail.asp?pr=CM0377&org=68&c=uk&lang=en
Slanetz & Bartley MF Media is available as a dehydrated culture media from various sources including
Other Sea Water Tests & Testing Instruments Not Suitable for Ocean Beach Water Sanitation Testing
Hanna Instruments sells a very wide range of testing instruments and kits world wide. Contact: HANNA instruments, Rhode Island
584 Park East Drive
Woonsocket, RI 02895
Phone: 800.426.6287 • 401.765.7500
Fax: 401.765.7575
Customer Service: custsvc@hannainst.com
Technical Support: tech@hannainst.com
General Sales: sales@hannainst.com
This company does not, however, provide test kits for bacterial contamination.
To consult with or purchase testing instruments from Hanna Instruments in Mexico see http://www.hannainst.com.mx/
Hydrometers, Refractometers and Salinity monitors are use for saltwater reef and marine aquarium testing but not for ocean swimming beach sanitation tests.
Photometer tests for use in Sea Water: a photometer such as the YSI Photometer can be used to test for certain sea water parameters of interest such as alkalinity, ammonia, calcium(hardness), chlorine, nitrates, nitrites, phosphate, permanganate, potassium, sulphates and sulfides.
This test is not suitable for checking directly for bacterial pathogens such as coliform or E. coli. - YSI Corp., 1725 Brannum Lane, Yellow Springs OH 45387, USA, TEl: 1-937-767-7241, TEl: 001-800-765-4974, Website: www.ysi.com, Email: environmental@ysi.com - retrieved 8/22/14, original source: http://www.ysi.com/media/pdfs/Photometer-Tests-in-Sea-Water.pdf
Watch out: salt water aquarium test kits are not suitable for testing ocean beach swimming water. Salt water aquarium test kits are intended to focus on salt water quality as it must be maintained for saltwater and reef aquarium keepers, addressing concentrations of ammonia, nitrite, nitrate, phosphate, pH, alkalilnity, calcium and copper.
The EPA has estimated that up to 3.5 million people become ill from contact with raw sewage from sanitary sewer overflows each year. 1 Many public health experts believe the number of illnesses caused by untreated sewage and other beach pollution sources may be much higher than is currently recognized because people who get sick from swimming in polluted recreational waters are not always aware of the cause of their illness and do not report it to doctors or local health officials.
Illnesses associated with polluted beach water include stomach flu, skin rashes, pinkeye, respiratory infections, meningitis, and hepatitis. Children are especially vulnerable, perhaps because they tend to submerge their heads more often than adults and are more likely to swallow water when swimming.
The Centers for Disease Control and Prevention concluded that the incidence of infections associated with recreational water use has steadily increased over the past several decades.
One study found that swimmers at polluted beaches in the Great Lakes region were more likely to have gastrointestinal illnesses than nonswimmers; another study found that fecal contamination at Los Angeles and Orange County beaches caused between 627,800 and 1,479,200 excess gastrointestinal illnesses each year.
This agency offers beach water testing advice and provides beach pollution information for beaches in many U.S. states. - retrieved 8/21/2014, original source: NRDC [Link defunct 2022/04/04]
Beach sanitation-safety advice from the NRDC
Whenever possible, swim at beaches that your research shows have the cleanest water, are carefully monitored, and have strict closure and advisory procedures.
If your beach is not monitored regularly, there are some things you can do to avoid swimming in polluted water:
If possible, choose beaches that are on open waters and away from urban areas. They frequently have cleaner water than beaches in developed areas or in enclosed bays and harbors with little water circulation.
Look for pipes along the beach that drain stormwater runoff from the streets, and don't swim near them. Avoid swimming in beach water that is cloudy or smells bad.
Keep your head out of the water.
Avoid swimming for at least 24 hours after it rains and 72 hours after heavy rains.
Contact local health officials if you suspect beach water contamination so that others can be protected from exposure.
WATERKEEPERS Baja Calfornias and its partners monitor water quality at beaches in this region all year long in the following communities: Tijuana, Loreto, Magdalena Bay, La Paz, Cabo San Lucas, San José del Cabo, Cabo Pulmo, and La Ribera.
WKBC utilizes a standardized region-wide protocol (see http://waterkeepersbaja.org/lo-que-hacemos/) for collecting and analyzing water samples, which is based on the Mexican government standard utilized for recreational beaches (see Comisión Federal para la Protección de Riesgos Sanitarios (COFEPRIS)).
Se recogen y analizan muestras de agua una vez al mes y los resultados se publican en Swim Guide México dentro de las 6 primeras horas de la obtención de resultados.
VERDE: Se marca una playa en color VERDE cuando en los resultados de una muestra se presenta un valor bajo del máximo permisible, 200 NMP (número más probable) de colonias de Enterococos por 100 ml de agua.
ROJO Se marca una playa en color ROJO cuando en los resultados de una muestra se presenta un valor por encima del máximo permisible, 200 NMP (número más probable) de colonias de Enterococos por 100 ml de agua.
Esa playa se mantiene marcada en ROJO por 72 horas después del resultado y se convierte a color GRIS si no hay más monitoreo. En caso de presentarse un valor por encima del máximo permisible (200 NMP), el programa local de WKBC notifica a las autoridades correspondientes:
Comisión Nacional del Agua (CNA) a través de la Comisión Federal para la Protección de Riesgos Sanitarios (COFEPRIS).
GRIS: Se marca una playa en color GRIS cuando no hay resultados actualizados o no hay información disponible.
Esta información es producto de un programa ciudadano de monitoreo de calidad del agua. La información oficial (cuando sea disponible) se encuentra aquí: http://www.semarnat.gob.mx/playas/playas-limpias/resultados de-calidad-de-agua-de-mar.
Water samples are collected once a month and results are posted on Swim Guide Mexico within 6 hours of obtaining results.
GREEN: A beach is marked GREEN when the results are under 200 NMP of Enterococus / 100 ML water
RED: A beach is marked RED when the results are above (and including) 200 NMP / 100 ML water.
A beach will remain RED for 72 hours and will convert to Grey until follow-up sampling occurs.
WKBC will alert the Mexican authorities: Comisión Nacional del Agua (CNA) y COFEPRIS.
GREY: A beach is marked GREY when there are no current results or there is no available information.
This information is the product of a citizen-monitoring program by members of WATERKEEPERS Baja Californias and its partner organizations. Official government results (when available) can be found here: http://www.semarnat.gob.mx/playas/playas-limpias/resultados de-calidad-de-agua-de-mar.
Privately-conducted Water testing at beaches in Mexico - [under research]. Also see (see Comisión Federal para la Protección de Riesgos Sanitarios (COFEPRIS)).
Last Best Beach, Playa Blanca,Mexico, Website: http://www.lastbestbeach.com/ [Beach water sanitation testing procuedure is under development]
Sea Water & Fresh Water Contaminant Testing Reference Standards
Here are two key water testing standards appropriate to tests used for swimming beaches where either fresh water or saltwater (marine beaches) are involved:
ASTM D5392-93 (2006) Standard Test Method for Isolation and Enumeration of Escherichia Coli in Water by the Two-Step Membrane Filter Procedure, Quoting ASTM:
Abstract
1.1 This test method describes a membrane filter (MF) procedure for the detection and enumeration of Escherichia coli, a bacterium found exclusively in the feces of humans and other warm-blooded animals.
The presence of these microorganisms in water is an indication of fecal pollution and the possible presence of enteric pathogens. These bacteria are found in water and wastewater in a wide range of densities. The detection limit of this procedure is one colony forming unit (CFU) per volume filtered.
1.2 This test method has been used successfully with temperate fresh and marine ambient waters, and wastewaters. It is the user"s responsibility to ensure the validity of this test method for waters of other types.
This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. For specific hazard statements, see Section 9.
ASTM D6503 - 99(2009)
Standard Test Method for Enterococci in Water Using Enterolert, Quoting: This test provides an easy and reliable method for the detection of enterococci in water within 24 h. For recreational water (fresh and marine) testing is performed to insure areas are safe for swimming. Enterolert also can be used for testing bottled water and drinking water.
1.1 This test method covers a simple procedure for the detection of enterococci in water and wastewater. It is based on IDEXX's patented Defined Substrate Technology (DST). This product, Enterolert, utilizes a nutrient indicator that fluoresces when metabolized.
It can detect these bacteria at one colony forming unit (CFU)/100 mL within 24 h. The presence of this microorganism in water is an indication of fecal contamination and the possible presence of enteric pathogens.
1.2 This test method can be used successfully with drinking water, source water, recreational (fresh and marine) water, and bottled water.
It is the user's responsibility to ensure the validity of this test method for waters of untested matrices.
1.3 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.
1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use.
It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
Our complete list of relevant standards for water testing and marine water testing for contaminants are located atReferences or Citations at the end of this article. See the "Click to Show or Hide Citations & References" link.
Clean Beach Water Testing Guides, Research, Information Sources for fresh water & salt water beaches & swimming ponds
Members of two bacteria groups, coliforms and fecal streptococci, are used as indicators of possible sewage contamination because they are commonly found in human and animal feces. Although they are generally not harmful themselves, they indicate the possible presence of pathogenic (disease-causing) bacteria, viruses, and protozoans that also live in human and animal digestive systems.
Therefore, their presence in streams suggests that pathogenic microorganisms might also be present and that swimming and eating shellfish might be a health risk. Since it is difficult, time-consuming, and expensive to test directly for the presence of a large variety of pathogens, water is usually tested for coliforms and fecal streptococci instead.
... The most commonly tested fecal bacteria indicators are total coliforms, fecal coliforms, Escherichia coli, fecal streptococci, and enterococci. All but E. coli are composed of a number of species of bacteria that share common characteristics such as shape, habitat, or behavior; E. coli is a single species in the fecal coliform group.
... EPA recommends E. coli as the best indicator of health risk from water contact in recreational waters; some states have changed their water quality standards and are monitoring accordingly.
... Enterococci are a subgroup within the fecal streptococcus group. Enterococci are distinguished by their ability to survive in salt water, and in this respect they more closely mimic many pathogens than do the other indicators. Enterococci are typically more human-specific than the larger fecal streptococcus group. EPA recommends enterococci as the best indicator of health risk in salt water used for recreation and as a useful indicator in fresh water as well.
EPA, WHAT MICROORGANISIMS COULD BE IN RECREATIONAL WATERr [PDF] U.S. EPA, Region 1: EPA New England, - retrieved 8/22/2014, original source epa.gov/region1/eco/beaches/qa.html , Excerpts:
What levels of indicator bacteria are considered acceptable:
Based on studies conducted in the 1980s, EPA has determined that a geometric mean (a measure of an overall average) in samples from recreational waters of less than 126 E. coli per 100 milliliters (ml) of fresh water or 35 enterococci per 100 ml of salt water is acceptable for protection of swimming.
The geometric mean should be calculated from more than five samples within the previous 30 days. If a single sample exceeds 235 E. coli per 100 ml in freshwater and 104 enterococci per 100 ml in salt water, EPA recommends that the beach be closed, or posted, for swimming until levels are lower.
(Some states, such as New Hampshire and Vermont, recommend that advisories be posted at more protective levels of indicator bacteria.) Because elevated fecal indicator bacteria are often associated with storm water runoff, some agencies post beaches preemptively if rainfall exceeds a set amount, based on site-specific studies.
... What laboratory methods are recommended for indicator bacteria for recreational waters?
There are several EPA-approved laboratory methods for measuring the abundance of E. coli or enterococci in recreational waters. These methods generally take 24 or 48 hours before a result is known. EPA recommends that 24 hour methods be used to minimize the time between sample collection and swimmer exposure.
EPA approved 24 hour membrane filtration methods are available at the Analytical Methods web site. Alternative popular 24 hour tests, such as the multiple-well fermentation tests for enterococci and E. coli (Enterolert® and Colilert®, respectively) manufactured by IDEXX Laboratories (Westbrook, ME) are also approved for recreational waters.
Koenders Water Solutions Inc.
3426 Saskatchewan Dr
Regina, Sk S4t 1h1
Sales: 1-877-888-7707
Service/support: 1-888-821-5533 Web: naturespondcare.com/ E-mail: Sales@naturespondcare.com , pond oxygenators, solar powered aeration systems
Koenders provides this helpful pond management guide:
Fender, Steve, POND MANAGEMENT [PDF] (2013) For more information contact: Steve Fender 50665 T.R. 220 Baltic, OH 43804 USA, retrieved ferom Koenders, op. cit. 2022/04/23 original source: naturespondcare.com/assets/uploads/downloads/Revised%20PondManagement_Fender.pdf
NH, BACTERIA in SURFACE WATERS [PDF] (2019) New Hapmshire Department of Environmental Services Fact Sheet, NHDES, 29 Hazen Dr., Concord HN 03301 USA, Tel: 603-271-3503, Web: des.nh.gov - retrieved 2023/10/04 origianl source: des.nh.gov/sites/g/files/ehbemt341/files/documents/2020-01/bb-14.pdf
Beach & Swimming Water Septic / Sewage Contamination Hazard Research
To protect bather health at recreational beaches, fecal indicator bacterial standards are used to monitor water quality, and waters exceeding the standards are subsequently closed to bathers. However beachgoers are also in contact with beach sands, the sanitary quality of which is not included within beach monitoring programs.
In fact, sands and sediments provide habitat where fecal bacterial populations may persist, and in some cases grow, in the coastal zone. Specific pathogens are less well studied in beach sands and sediments, but there is a body of evidence that they too may persist in these environments.
This paper reviews the current state of knowledge regarding the abundance and distribution of fecal indicator bacteria and pathogens in beach sands of diverse climatological regions, and at beaches subjected to varied levels of anthropogenic impact.
In all regions fecal indicator bacteria are nearly ubiquitous in beach sands, and similar relationships emerge between fecal indicator abundance in dry sand, submerged sands, and water. Taken together, these studies contextualize a potential public health issue and identify research questions that must be addressed in order to support future policy decisions.
...
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In addition to any citations in the article above, a full list is available on request.
Thanks to Alex Astachovsky from Simpletek for assistance with describing the company's water testing products and alternatives. Contact:
Simpltek
8 Byron Road
Commack, NY 11725
631-864-0099
http://www.simpltek.com
alex@simpltek.com
Clean Beach Water Testing Guides, Research, Information Sources for fresh water & salt water beaches
Abdelzaher, Amir M., Mary E. Wright, Cristina Ortega, Helena M. Solo-Gabriele, Gary Miller, Samir Elmir, Xihui Newman et al. "Presence of pathogens and indicator microbes at a non-point source subtropical recreational marine beach." Applied and environmental microbiology 76, no. 3 (2010): 724-732.
Anderson, IRIS C., M. Rhodes, and H. Kator. "Sublethal stress in Escherichia coli: a function of salinity." Applied and environmental microbiology 38, no. 6 (1979): 1147-1152.
Armon, R., and Y. Kott. "Bacteriophages as indicators of pollution." Critical reviews in environmental science and technology 26, no. 4 (1996): 299-335.
Boehm, Alexandria B., Jed A. Fuhrman, Robert D. Mrše, and Stanley B. Grant. "Tiered approach for identification of a human fecal pollution source at a recreational beach: case study at Avalon Bay, Catalina Island, California." Environmental science & technology 37, no. 4 (2003): 673-680.
Bordalo, A. A. "Faecal coliform recovery in two standard media along an estuarine gradient." Water Research 28, no. 11 (1994): 2331-2334.
ColweW, R. R., and J. Kaper. "Vibrio species as bacterial indicators of potential health hazards associated with water." In Bacterial indicators/health hazards associated with water: a symposium, vol. 1976, p. 115. American Society for Testing and Materials, 1977.
Darcan, Cihan, Resit Ozkanca, Oende Idil, and Ken P. Flint. "Viable but non-culturable state (VBNC) of Escherichia coli related to EnvZ under the effect of pH, starvation and osmotic stress in sea water." Polish journal of microbiology/Polskie Towarzystwo Mikrobiologow= The Polish Society of Microbiologists 58, no. 4 (2008): 307-317.
Dufour, A. P. "Escherichia coli: the fecal coliform." Bacterial indicators/health hazards associated with water 635 (1977): 48.Abstract: The use of Escherichia coli as an indicator of fecal pollution and the significance of its presence in surface waters was reviewed. A minidefinition which identifies this organism at least 95 percent of the time was proposed. Membrane filtration and most probable number methods for the enumeration of E. coli were discussed.
Dufour, A. P., and V. J. Cabelli. "Membrane filter procedure for enumerating the component genera of the coliform group in seawater." Applied microbiology 29, no. 6 (1975): 826-833.
Elliot, Elisa L., and Rita R. Colwell. "Indicator organisms for estuarine and marine waters." FEMS Microbiology Letters 32, no. 2 (1985): 61-79.
Evans, T. M., C. E. Waarvick, RAMON J. Seidler, and M. W. LeChevallier. "Failure of the most-probable-number technique to detect coliforms in drinking water and raw water supplies." Applied and environmental microbiology 41, no. 1 (1981): 130-138.
Feng, Peter, Stephen D. Weagant, Michael A. Grant, William Burkhardt, Molluscan Shellfish, and Bottled Water. "BAM: Enumeration of Escherichia coli and the Coliform Bacteria." Bacteriological Analytical Manual (2002).
Feng, P. C. S., and Paul A. Hartman. "Fluorogenic assays for immediate confirmation of Escherichia coli." Applied and environmental microbiology 43, no. 6 (1982): 1320-1329. Abstract: Rapid assays for Escherichia coli were developed by using the compound 4-methylumbelliferone glucuronide (MUG), which is hydrolyzed by glucuronidase to yield a fluorogenic product. The production of glucuronidase was limited to strains of E. coli and some Salmonella and Shigella strains in the family Enterobacteriaceae.
For immediate confirmation of the presence of E. coli in most-probable-number tubes, MUG was incorporated into lauryl tryptose broth at a final concentration of 100 micrograms/ml. Results of both the presumptive test (gas production) and the confirmed test (fluorescence) for E. coli were obtained from a variety of food, water, and milk samples after incubation for only 24 h at 35 degrees C. Approximately 90% of the tubes showing both gas production and fluorescence contained fecal coliforms (they were positive in EC broth incubated at 45 degrees C). Few false-positive reactions were observed. The lauryl tryptose broth-MUG-most-probable-number assay was superior to violet red bile agar for the detection of heat- and chlorine-injured E. coli cells. Anaerogenic strains produced positive reactions, and small numbers of E. coli could be detected in the presence of large numbers of competing bacteria.
The fluorogenic assay was sensitive and rapid; the presence of one viable cell was detected within 20 h. E. coli colonies could be distinguished from other coliforms on membrane filters and plates of violet red bile agar if MUG was incorporated into the culture media. A rapid confirmatory test for E. coli that is amenable to automation was developed by using microtitration plates filled with a nonselective medium containing MUG. Pure or mixed cultures containing E. coli produced fluorescence within 4 h (most strains) to 24 h (a few weakly positive strains).
Figueras, M. J., F. Polo, I. Inza, and J. Guarro. "Past, present and future perspectives of the EU bathing water directive." Marine pollution bulletin 34, no. 3 (1997): 148-156.
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Halley, A. W., and Bernard J. Dutka, eds. Bacterial Indicators/health Hazards Associated with Water. Vol. 635. ASTM International, 1977.
Olson, BETTY H. "Enchanced accuracy of coliform testing in seawater by a modification of the most-probable-number method." Applied and environmental microbiology 36, no. 3 (1978): 438-444.
Gauthier, Michel J., Patrick M. Munro, and Soussan Mohajer. "Influence of salts and sodium chloride on the recovery ofEscherichia coli from seawater." Current Microbiology 15, no. 1 (1987): 5-10.
Gauthier, M. J., V. M. Torregrossa, M. C. Babelona, R. Cornax, and J. J. Borrego. "An Intercalibration Study of the Use of 4-Methylumbelliferyl-β-D-Glucuronide for the Specific Enumeration of< i> Escherichia coli</i> in Seawater and Marine Sediments." Systematic and applied microbiology 14, no. 2 (1991): 183-189.
Jagals, P., and W. O. K. Grabow. "An evaluation of sorbitol-fermenting bifidobacteria as specific indicators of human faecal pollution of environmental water." WATER SA-PRETORIA- 22 (1996): 235-238.
Kinzelman, Julie, Clement Ng, Emma Jackson, Stephen Gradus, and Robert Bagley. "Enterococci as indicators of Lake Michigan recreational water quality: Comparison of two methodologies and their impacts on public health regulatory events." Applied and Environmental Microbiology 69, no. 1 (2003): 92-96.
LaBelle, Raymond L., Charles P. Gerba, Sagar M. Goyal, Joseph L. Melnick, Irina Cech, and Gregory F. Bogdan. "Relationships between environmental factors, bacterial indicators, and the occurrence of enteric viruses in estuarine sediments." Applied and environmental microbiology 39, no. 3 (1980): 588-596.
Lee, Kyu-Ho, and Edward G. Ruby. "Detection of the light organ symbiont, Vibrio fischeri, in Hawaiian seawater by using lux gene probes." Applied and environmental microbiology 58, no. 3 (1992): 942-947.
Mates, A. "The significance of testing for Pseudomonas aeruginosa in recreational seawater beaches." Microbios 71, no. 287 (1991): 89-93.
Mates, A., and M. Schaffer. "Quantitative determination of Escherichia coli from faecal coliforms in seawater." Microbios 53, no. 216-217 (1987): 161-165.
Medema, G. J., M. Bahar, and F. M. Schets. "Survival of< i> Cryptosporidium parvum</i>,< i> Escherichia coli</i>, faecal enterococci and< i> Clostridium perfringens</i> in river water: influence of temperature and autochthonous microorganisms." Water Science and Technology 35, no. 11 (1997): 249-252.
Mena, Kristina D., and Charles P. Gerba. "Risk assessment of Pseudomonas aeruginosa in water." In Reviews of Environmental Contamination and Toxicology Vol 201, pp. 71-115. Springer US, 2009.
Miescier, John J., and Victor J. Cabelli. "Enterococci and other microbial indicators in municipal wastewater effluents." Journal (Water Pollution Control Federation) (1982): 1599-1606.
Miescier, JOHN J., VIRGIL E. Carr, JOHN F. Musselman, and SANTO A. Furfari. "Fecal coliform methods for examination of sea water: interlaboratory evaluation of split sample analysis." Journal-Association of Official Analytical Chemists 61, no. 4 (1978): 772-778.
Palmer, Carol J., Yu-Li Tsai, A. Lee Lang, and Louis R. Sangermano. "Evaluation of colilert-marine water for detection of total coliforms and Escherichia coli in the marine environment." Applied and Environmental Microbiology 59, no. 3 (1993): 786-790. - retrieved 8/21/14, original source http://aem.asm.org/content/59/3/786.full.pdf Note:
Updated information and services can be found at:
http://aem.asm.org/content/59/3/786 Abstract:
A test that allows for early detection of fecaly contaminated coastal water would enhance public health protection. Colilert-Marine Water (Colilert-MW; Environetics, Branford, Conn.) is a rapid 24-h test that has recently been developed to detect total coliforms and Escherichia coli in coastal water. We performed a premarketing evaluation of the Colilert-MW product, testing it in parallel with the multiple tube fermentation (MTF) method for 86 coastal water samples in southern California. Statistic alanalysis was performed by using paired t tests and linear regression. Bacterial isolates were evaluated by bio chemical and genetic analysis.The results of this study showed a strong correlation between the traditional MTF and the Colilert-MW method for detection of total coliforms (r= 0.95) and E.coli (r= 0.89) inocean water samples. Paired t-test results indicated that the Colilert-MW and MTF were equivalent in detecting E.coli and tha tthe Colilert-MW may be more sensitive in the detection of total coliforms. We conclude that Colilert-MW would be a useful tool with which to monitor coastal beach water. [Copy on file - Ed. ]
Pommepuy, M., M. Butin, A. Derrien, M. Gourmelon, R. R. Colwell, and M. Cormier. "Retention of enteropathogenicity by viable but nonculturable Escherichia coli exposed to seawater and sunlight." Applied and Environmental Microbiology 62, no. 12 (1996): 4621-4626.
Reeves, Ryan L., Stanley B. Grant, Robert D. Mrse, Carmen M. Copil Oancea, Brett F. Sanders, and Alexandria B. Boehm. "Scaling and management of fecal indicator bacteria in runoff from a coastal urban watershed in southern California." Environmental science & technology 38, no. 9 (2004): 2637-2648.
Robertson, W. J., and Richard S. Tobin. "The relationship between three potential pathogens and pollution indicator organisms in Nova Scotian coastal waters." Canadian journal of microbiology 29, no. 10 (1983): 1261-1269.
Santoro, Alyson E., Christopher A. Francis, Nicholas R. De Sieyes, and Alexandria B. Boehm. "Shifts in the relative abundance of ammonia‐oxidizing bacteria and archaea across physicochemical gradients in a subterranean estuary." Environmental Microbiology 10, no. 4 (2008): 1068-1079.
Sartory, D. P. "Membrane filtration enumeration of faecal clostridia and< i> clostridium perfringens</i> in water." Water Research 20, no. 10 (1986): 1255-1260.
Seyfried, PATRICIA L., Richard S. Tobin, NANCY E. Brown, and Peter F. Ness. "A prospective study of swimming-related illness. II. Morbidity and the microbiological quality of water." American journal of public health 75, no. 9 (1985): 1071-1075.
Sinton, L. W., R. K. Finlay, and D. J. Hannah. "Distinguishing human from animal faecal contamination in water: a review." New Zealand Journal of Marine and Freshwater Research 32, no. 2 (1998): 323-348.
Wheeler Alm, Elizabeth, Janice Burke, and Anne Spain. "Fecal indicator bacteria are abundant in wet sand at freshwater beaches." Water research 37, no. 16 (2003): 3978-3982.
Xu, Huai-Shu, N. Roberts, F. L. Singleton, R. W. Attwell, D. J. Grimes, and R. R. Colwell. "Survival and viability of nonculturableEscherichia coli andVibrio cholerae in the estuarine and marine environment." Microbial Ecology 8, no. 4 (1982): 313-323.
Yamahara, Kevan M., Blythe A. Layton, Alyson E. Santoro, and Alexandria B. Boehm. "Beach sands along the California coast are diffuse sources of fecal bacteria to coastal waters." Environmental science & technology 41, no. 13 (2007): 4515-4521.
Sea Water & Fresh Water Contaminant Testing Reference Standards
ASTM D3352-08a Standard Test Method for Strontium Ion in Brackish Water, Seawater, and Brines
ASTM D3651-11 Standard Test Method for Barium in Brackish Water, Seawater, and Brines, Quoting: Since water containing acid-soluble barium compounds is known to be toxic, this test method serves the useful purpose of determining the barium in brackish water, seawater, and brines.
ASTM D5392-93 (2006) Standard Test Method for Isolation and Enumeration of Escherichia Coli in Water by the Two-Step Membrane Filter Procedure, Quoting ASTM:
Abstract
1.1 This test method describes a membrane filter (MF) procedure for the detection and enumeration of Escherichia coli, a bacterium found exclusively in the feces of humans and other warm-blooded animals. The presence of these microorganisms in water is an indication of fecal pollution and the possible presence of enteric pathogens. These bacteria are found in water and wastewater in a wide range of densities. The detection limit of this procedure is one colony forming unit (CFU) per volume filtered.
1.2 This test method has been used successfully with temperate fresh and marine ambient waters, and wastewaters. It is the user"s responsibility to ensure the validity of this test method for waters of other types.
This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. For specific hazard statements, see Section 9.
ASTM D3561-11 Standard Test Method for Lithium, Potassium, and Sodium Ions in Brackish Water, Seawater, and Brines by Atomic Absorption Spectrophotometry
ASTM D3868-09 Standard Test Method for Fluoride Ions in Brackish Water, Seawater, and Brines
ASTM D3869-09 Standard Test Methods for Iodide and Bromide Ions in Brackish Water, Seawater, and Brines
ASTM D3875-08, Standard Test Method for Alkalinity in Brackish Water, Seawater, and Brines. Quoting ASTM: Alkalinity as carbonate and bicarbonate of saline water is very important in chemical waterflooding or tertiary recovery processes for recovering petroleum. Alkaline waters offer better wetting to the formation rock and improve oil release. As an additional benefit, ions that provide alkalinity adsorb on rock surfaces occupying adsorption sites and decrease the loss of recovery chemical by adsorption. Determination of alkalinity in waters used in tertiary recovery processes is therefore very important.
An alkalinity value is necessary in the calculation of carbonate scaling tendencies of saline waters. It is also necessary to determine the alkalinity if the ionic balance of a water analysis is to be used as a check of the reliability of the analysis. - Retrieved 8/22/14 original source http://www.astm.org/Standards/D3875.htm
ASTM D3986-11 Standard Test Method for Barium in Brines, Seawater, and Brackish Water by Direct-Current Argon Plasma Atomic Emission Spectroscopy
ASTM D4130-08 Standard Test Method for Sulfate Ion in Brackish Water, Seawater, and Brines
ASTM D4458-09 Standard Test Method for Chloride Ions in Brackish Water, Seawater, and Brines
ASTM D6503 - 99(2009) Standard Test Method for Enterococci in Water Using Enterolert, Quoting: This test provides an easy and reliable method for the detection of enterococci in water within 24 h. For recreational water (fresh and marine) testing is performed to insure areas are safe for swimming. Enterolert also can be used for testing bottled water and drinking water.
1.1 This test method covers a simple procedure for the detection of enterococci in water and wastewater. It is based on IDEXX's patented Defined Substrate Technology (DST). This product, Enterolert, utilizes a nutrient indicator that fluoresces when metabolized. It can detect these bacteria at one colony forming unit (CFU)/100 mL within 24 h. The presence of this microorganism in water is an indication of fecal contamination and the possible presence of enteric pathogens.
1.2 This test method can be used successfully with drinking water, source water, recreational (fresh and marine) water, and bottled water. It is the user's responsibility to ensure the validity of this test method for waters of untested matrices.
1.3 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.
1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
Standard Methods for the Examination of Water and Wastewater, 1-877-574-1233
1-703-684-2469 (outside U.S. / Canada)
support@standardmethods.org, (Contact with questions regarding technical information; Standard Methods news)
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sposavec@awwa.org, Website: http://www.standardmethods.org/, Quoting: Since 1905, Standard Methods for the Examination of Water and Wastewater has represented "the best current practice of American water analysts." This comprehensive reference covers all aspects of water and wastewater analysis techniques. Standard Methods is a joint publication of the American Public Health Association (APHA), the American Water Works Association (AWWA), and the Water Environment Federation (WEF).
[1]US EPA, "Water: Basic Information about Regulated Drinking Water Contaminants", retrieved 8/27/2013, original source http://water.epa.gov/drink/contaminants/basicinformation/disinfectants.cfm
CHLORINE IN SEPTIC WASTEWATER - Septic Tank Maintenance: Chlorine in Septic Systems - Septic Tank Maintenance Advice
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Handbook of Disinfectants and Antiseptics, Joseph M. Ascenzi (Editor), CRC, 1995, ISBN-10: 0824795245 ISBN-13: 978-0824795245 "The evaluation of chemical germicides predates the golden age of microbiology..." -
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