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MOLD: A COMPLETE GUIDE to TEST CLEAN PREVENT

AIR CLEANER PURIFIER TYPES
AIR FILTERS for HVAC SYSTEMS
AIR POLLUTANTS, COMMON INDOOR
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ALLERGEN TESTS for BUILDINGS
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ATTORNEYS and EXPERT WITNESSES

BASEMENT MOLD
BATHROOM MOLD
BIBLIOGAPHY ENVIRONMENTAL HEALTH, MOLD, IAQ
BIOLOGICAL POLLUTANTS

CAR MOLD CONTAMINATION
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CAT DANDER in BUILDINGS
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CONDENSATION or SWEATING PIPES, TANKS
CPSC Indoor Air Pollution Book Online Copy

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FIBERGLASS HAZARDS
FLOOD DAMAGE ASSESSMENT, SAFETY & CLEANUP
FLOODS IN BUILDINGS-mold
FORMALDEHYDE HAZARDS

GAS EXPOSURE EFFECTS, TOXIC

HUMIDITY CONTROL & TARGETS INDOORS
HOUSE DUST ANALYSIS

INDOOR AIR QUALITY IMPROVEMENT GUIDE
INSULATION MOLD

MILDEW REMOVAL & PREVENTION
MOISTURE CONTROL in BUILDINGS
MOLD GROWTH on SURFACES, TABLE OF
MYCOPHOBIA, STAINS MISTAKEN for MOLD

NOISE / SOUND DIAGNOSIS & CURE

ODORS GASES SMELLS, DIAGNOSIS & CURES
OZONE HAZARDS
OZONE for MOLD OR ODORS

RADON HAZARD TESTS & MITIGATION

SAFETY HAZARDS GUIDE
SICK HOUSE IAQ QUESTIONNAIRE
SMELL PATCH TEST to Track Down Odors
STAIN DIAGNOSIS on BUILDING INTERIORS

VENTILATION in BUILDINGS

More Information

Mold culture plate (C) Daniel FriedmanMold Identification Photographs of Mold Growing on Petri Dishes or Mold Culture Plates or Settlement Plates
     

  • Mold Photos in Petri Dishes - Not so Useful for Environmental Sampling and Mold Identification - What Level of Magnification is Needed to Identify Mold?
  • Can photos provide reliable identification of mold on culture plates or petri dishes?
  • Limitations of mold cultures as a "mold test kit"
  • Usefulness of culturing for the identification and study of mold
  • 90% error rate in capture of mold genera/species with some mold test methods
  • Do all molds grow on culture?
  • Do all mold spores settle out of air at the same rate onto culture plates?
  • Are all mold spores equally important in mold samples?
  • MOLD CULTURE TEST KIT VALIDITY - separate article
    • MOLD CULTURE PHOTOS
    • MOLD CULTURE SAMPLING METHOD - separate article
    • MOLD CULTURE TEST ERRORS - separate article
  • Questions & Answers about using culture tests for building mold contamination
  • References

Click to Show or Hide Related Topics

  • ACCURACY OF VARIOUS MOLD TEST METHODS - home
  • ACCURACY vs PRECISION of MEASUREMENTS
  • AIR TEST SAMPLING CASSETTE STUDY
  • AIRBORNE MOLD COUNT NUMBER GUIDE
  • AIRBORNE MOLD COUNT VALIDITY
  • AIRBORNE PARTICLE ANALYSIS METHODS
  • CONCENTRATION BURSTS of Mold Spores
  • CULTURE PLATE Test Errors, Mold
  • DUST SAMPLING PROCEDURE
  • FALSE NEGATIVEW Results in Mold Tests
  • FORENSIC & IAQ FIELD IAQ EQUIPMENT SOP - home
  • FORENSIC & IAQ LAB MICROSCOPE TECHNIQUES
  • FORENSIC LAB TECHNICAL PROCEDURES
  • HIDDEN MOLD, HOW TO FIND
  • INDOOR vs OUTDOOR Airborne Spore Counts
  • MOLD LEVEL IN AIR, VALIDITY
  • MOLD STANDARDS
  • MOLD TESTING METHOD VALIDITY
  • PARTICLE & MOLD LEVELS in DUCTWORK
  • SLIDE PREPARATION, MICROSCOPE
  • VALIDITY of MOLD TESTING METHODS
  • VARIATION IN AIRBORNE Particle Levels, Causes of
  • VARIATION IN AIRBORNE Particle Levels vs Sampler Height
  • VARIATION IN AIRBORNE PARTICLE COUNTS, Extent of
InspectAPedia tolerates no conflicts of interest. We have no relationship with advertisers, products, or services discussed at this website.

Photographs of mold culture test kit results: this article illustrates common results of mold test kits that use a culture medium, and along with several companion articles listed here, it explains the availability and usefulness of eye-level or low-power magnification photographs of mold growing on mold culture plates, settlement plates, and mold test kits to try to identify indoor mold contamination.

Green links show where you are. © Copyright 2013 InspectAPedia.com, All Rights Reserved. Author Daniel Friedman.

Mold Photos in Petri Dishes - Not so Useful for Environmental Sampling and Mold Identification - What Level of Magnification is Needed to Identify Mold?

Mold culture (C) Daniel FriedmanFollowing the list of some of our online guides to building mold just below, we include photographs of what mold looks like when growing on culture media commonly used in home test kits for mold.

In this article series we discuss the validity of nearly all of the popular mold testing methods currently in use, pointing out the strengths and weakness of each approach to mold sampling in the indoor environment, beginning with air sampling for airborne mold levels indoors.

The use, accuracy, and reliability of mold culture test kits for screening buildings for mold contamination are discussed at MOLD CULTURE TEST KIT VALIDITY and MOLD CULTURE SAMPLING METHOD Also see Mold Culture Plate Test Errors.

 

Mold Test Kit Culture Plates: Why Can't I Find More Photos of Mold in Petri Dishes?

Mold petri dish (C) Daniel FriedmanQuestion:

I was disappointed as there were no photos at all of petri dish examples of mold, and this is the way most of us out here will be testing for mold. So, how do I explore what mold I have in my petri dish test? I have quarter sized discs of black/dark green mold growing. I did the airborne mold test.

My dog is always coughing and I am in pretty good health but feel a slight "tug" in my breathing, a slight heaviness in my lungs but not bad. I rent my apartment and my landlord is a total tool.

What can I do financially and health wise to explore my situation? Thanks for any tips!!! - Tony K

Reply: Microscopic Examination of Mold is Necessary for Reliable Identification

By Eye Examination of Culture Plates or Petri Dishes to Identify Mold?

The short answer is that you cannot reliably identify what mold is found in a petri dish simply by looking at some photos or color charts. Some mold genera or species might be ruled "out" or "possible" but expert examination of the sample using high-powered microscopy (or another definitive method) is needed.

About what you can do about mold, take a look at MOLD EXPERT, WHEN TO HIRE - for help in deciding if your situation honestly merits hiring an expert. Then see MOLD ACTION GUIDE - WHAT TO DO ABOUT MOLD. If you are using mold culture plates, because mold test validity and mold test accuracy are often confused, see Cultures to "Test for Mold". Also see MOLD GROWTH on SURFACES for an index of what mold genera/species are frequently found on various building surfaces and materials.

Mold petri dish (C) Daniel Friedman

We do have some photos of mold in petri dishes posted just above and online, at other of our online articles about the role and limitations of using mold cultures as "home test kits" at Cultures to "Test for Mold". (You'll see there that what grows in culture is not necessarily the dominant or most significant mold that is present in a building.)

Traditionally, petri dish or culture plate photos were included in early mold taxonomy texts, where color and texture of mold growth at that scale assisted in identification of cultures of a known genera down to species level.

These were photos of mold cultured in laboratories where it is sometimes possible to separate a genera of mold (Aspergillus sp.) into species or groups of species (Aspergillus niger) based on color and other macro-characteristics.


Penicillium in culture (C) Daniel Friedman


In the closeup of a mold culture petri dish growth shown in our photo at above-right, high-powered microscopic examination was necessary to identify Penicillium sp (photo at left) as one of the several mold genera growing among these green, gray,and dark gray colored mold colonies.

Sometimes we can make a pretty good guess about mold identification by the naked eye, if we see a particular color and texture of mold on a particular surface. For example this photo of mold on an orange is showing what is most likely a species of Penicillium.

But in general that "by eye" mold identification approach is not reliable.

Stereoscopic Microscope Photos of Mold to Identify It?

Fuligo septic (C) Daniel Friedman

An "in-between" level of magnification, between using the naked eye to look at mold culture growing in a petri dish and using a high powered microscope is the use of a stereo microscope to magnify mold growth on surfaces such as on culture media in a petri dish.

For example, our stereoscopic microscopy photo of Fuligo septica (left) is characteristic of that particular fungus.

Stereoscopic mold photos are often beautiful (like this stereoscopic photo of Stemonitis mold growth structures taken in our lab) and may be helpful in identifying a mold genera. Here, for contrast, is a high power microscope photo of Stemonitis mold spores.

But stereoscopic magnification is inadequate for reliable mold identification.

High Powered Microscopic Identification of Mold Spores

Fuligo septic  (C) Daniel Friedman


For environmental samples in which we need to identify mold genera/species or other particles, it's a different story.

As we operate a forensic lab that processes lots of materials including mold, collected by various means, we see that while petri dish photos are pretty, they are not diagnostic, nor can they be used alone for mold identification at that scale.

We need to examine mold structures and spores at 300x to 1200x to actually identify genera/species reliably. See MOLD BY MICROSCOPE for examples.

The Fuligo septica mold spores in our photo provide very different information than what we can get by eye looking at a mold culture plate or petri dish.

Also see more photos at MOLD ATLAS & PARTICLES INDEX and our Alphabetic Index to Mold Genera or Species

At MOLD "TESTING" vs. MOLD "PROBLEM IDENTIFICATION" we discuss the question of what sorts of mold testing are most useful and which are actually diagnostic, giving information about the presence of a mold problem with enough information that you know what to do about it.

Additional Comments on Mold Culture Identification

Mold cultures involve the collection of particles by air sampling pump, by gravity settlement, or by lift from a surface using a swab or tape. Some sampling equipment (Anderson™ spore traps) can collect spores directly into a petri dish of culture medium, and are used for "viable spore sampling in air."

Mold Culturing is useful for genera speciation once you have collected a single or dominant sample whose importance (frequency in the building) you already know. As a "home test kit" for the presence of problematic mold in a building this is an unreliable method, as we describe below at "shortcomings."

Mold culture (C) Daniel Friedman Mold culture (C) Daniel Friedman

Our lab photo (above left) shows two different mold colonies growing on a culture plate where individual spores settled out of the air onto this surface.


Frequently Asked Questions (FAQs) about photographs of mold growth on culture plates

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Technical Reviewers & References

Related Topics, found near the top of this page suggest articles closely related to this one.

  • MOLD ATLAS & PARTICLES INDEX - A-to-Z Mold Atlas of Indoor Clinical Mold, Pathogens, Allergens & Other Indoor Particles
  • MOLD APPEARANCE - WHAT MOLD LOOKS LIKE - What Does Black or Dark Indoor Mold Look Like? Black Mold spores in the Home - a Photo ID Library. What toxic black mold or other indoor mold looks like in buildings.
  • MOLD GROWTH ON SURFACES, PHOTOS - What Does Mold Look Like on Various Materials & Surfaces? An extensive photographic guide to mold as it is found growing on various building materials & surfaces. Also see MOLD GROWTH on SURFACES, TABLE OF - a Table of Kinds of Mold Growth Found on Building Surfaces, lists mold genera/species most often found on specific building surfaces, materials, or contents
  • MOLD in the PETRI DISH, PHOTOS. - Photographs of Mold Growing on Petri Dishes or Mold Culture Plates or Settlement Plates
  • MOLD BY MICROSCOPE - Mold spores under the Microscope - a Photo ID Library for detection and identification of toxic or other mold
  • MOLD RELATED ILLNESS SYMPTOMS - Mold Related Illness: Index of Symptoms. Readers should not rely on this document for medical diagnosis and instead should consult with their physician or with a specialist such as a medical toxicologist
  • Mark Cramer Inspection Services Mark Cramer, Tampa Florida, Mr. Cramer is a past president of ASHI, the American Society of Home Inspectors and is a Florida home inspector and home inspection educator. (727) 595-4211 mark@BestTampaInspector.com
  • Thanks to Tony K for discussing mold in petri dishes and indoor sampling or mold testing using cultures - July 2010.

Books & Articles on Building & Environmental Inspection, Testing, Diagnosis, & Repair

  • Our recommended books about building & mechanical systems design, inspection, problem diagnosis, and repair, and about indoor environment and IAQ testing, diagnosis, and cleanup are at the InspectAPedia Bookstore. Also see our Book Reviews - InspectAPedia.
  • Home Reference Book - Carson DunlopThe Home Reference Book - the Encyclopedia of Homes, Carson Dunlop & Associates, Toronto, Ontario, 25th Ed., 2012, is a bound volume of more than 450 illustrated pages that assist home inspectors and home owners in the inspection and detection of problems on buildings. The text is intended as a reference guide to help building owners operate and maintain their home effectively. Field inspection worksheets are included at the back of the volume. Special Offer: For a 10% discount on any number of copies of the Home Reference Book purchased as a single order. Enter INSPECTAHRB in the order payment page "Promo/Redemption" space. InspectAPedia.com editor Daniel Friedman is a contributing author.

    Or choose the The Home Reference eBook for PCs, Macs, Kindle, iPad, iPhone, or Android Smart Phones. Special Offer: For a 5% discount on any number of copies of the Home Reference eBook purchased as a single order. Enter INSPECTAEHRB in the order payment page "Promo/Redemption" space.

  • GO TO Carson Dunlop's Home Study Course Information - How to Become a Home Inspector: Carson Dunlop's nationally recognized Home Study Course, selected by ASHI the American Society of Home Inspectors and other professionals and associations. This website author is a contributor to this course.
  • GO TO Carson Dunlop's Home Study Course Information - How to Become a Home Inspector: Carson Dunlop's nationally recognized Home Study Course, selected by ASHI the American Society of Home Inspectors and other professionals and associations. This website author is a contributor to this course.
  • GO TO Carson Dunlop's Home Study Course Information - How to Become a Home Inspector: Carson Dunlop's nationally recognized Home Study Course, selected by ASHI the American Society of Home Inspectors and other professionals and associations. This website author is a contributor to this course.
    Building inspection education & report writing systems from Carson, Dunlop & Associates Ltd
  • Environmental Health & Investigation Bibliography - our technical library on indoor air quality inspection, testing, laboratory procedures, forensic microscopy, etc.
  • Adkins and Adkins Dictionary of Roman Religion discusses Robigus, the Roman god of crop protection and the legendary progenitor of wheat rust fungus.
  • Kansas State University, department of plant pathology, extension plant pathology web page on wheat rust fungus: see http://www.oznet.ksu.edu/path-ext/factSheets/Wheat/Wheat%20Leaf%20Rust.asp
  • "A Brief Guide to Mold, Moisture, and Your Home", U.S. Environmental Protection Agency US EPA - includes basic advice for building owners, occupants, and mold cleanup operations. See http://www.epa.gov/mold/moldguide.htm
  • US EPA - Mold Remediation in Schools and Commercial Building [Copy on file at /sickhouse/EPA_Mold_Remediation_in_Schools.pdf ] - US EPA
  • US EPA - Una Breva Guia a Moho - Hongo [Copy on file as /sickhouse/EPA_Moho_Guia_sp.pdf - en Espanol
  • "A Brief Guide to Mold, Moisture, and Your Home", U.S. Environmental Protection Agency US EPA - includes basic advice for building owners, occupants, and mold cleanup operations. See http://www.epa.gov/mold/moldguide.htm
  • "Disease Prevention Program for Certain Vegetable Crops," David B. Langston, Jr., Extension Plant Pathologist - Vegetables, University of Georgia (PDF document) original source: www.reeis.usda.gov/web/crisprojectpages/209797.html
  • "Disease Prevention in Home Vegetable Gardens," Patricia Donald, Department of Plant Microbiology and Pathology, Lewis Jett
    Department of Horticulture, University of Missouri Extension - extension.missouri.edu/publications/DisplayPub.aspx?P=G6202
  • "Management of Powdery Mildew, Leveillula taurica, in Greenhouse Peppers," Ministry of Agriculture and Lands, British Columbia - Original source: www.agf.gov.bc.ca/cropprot/peppermildew.htm
  • Fifth Kingdom, Bryce Kendrick, ISBN13: 9781585100224, is available from the InspectAPedia online bookstore - we recommend the CD-ROM version of this book. This 3rd/edition is a compact but comprehensive encyclopedia of all things mycological. Every aspect of the fungi, from aflatoxin to zppspores, with an accessible blend of verve and wit. The 24 chapters are filled with up-to-date information of classification, yeast, lichens, spore dispersal, allergies, ecology, genetics, plant pathology, predatory fungi, biological control, mutualistic symbioses with animals and plants, fungi as food, food spoilage and mycotoxins.
  • Fungi, Identifying Filamentous, A Clinical Laboratory Handbook, Guy St-
  • US EPA: Mold Remediation in Schools and Commercial Building [Copy on file at /sickhouse/EPA_Mold_Remediation_in_Schools.pdf ] - US EPA
  • Mycology, Fundamentals of Diagnostic, Fran Fisher, Norma B. Cook, W.B. Saunders Co. 1998, ISBN 0-7216-5006-6 (buy this book at Amazon)
  • ...
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