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Stachybotrys spores (left) and structure (right)Airborne Particle Calculations
How to calculate airborne particle concentrations for mold, IAQ, allergens, or other forensic particle studies - Mold Test Laboratory SOPs
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  • AIRBORNE PARTICLE ANALYSIS METHODS - Detailed procedure for computing the count of particles per cubic meter of air - indoor air quality and mold test lab procedures
    • Particles per Cubic Meter
    • Equipment Calibration
    • Microscope calibration
    • Quantitative Particle Counts
    • Lab Chemicals for Particle Samples
    • Qualitative Vacuum Sample Analysis
    • Qualitative Surface Analysis
    • Sample Microscope Calculations
    • Example Count w/ Allergenco Sampler
    • Alternative count procedure
    • Counting Airborne Mold Concentrations
    • Airborne Mold or Level Reporting
    • Equipment Trace Dimensions
  • Questions & answers abouthow to calculate airborne mold or other airborne particle levels expressed as n particles per cubic meter of air
  • References

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  • INDOOR AIR QUALITY IMPROVEMENT GUIDE - home
  • ACCURACY OF VARIOUS MOLD TEST METHODS - home
  • AIR POLLUTANTS, COMMON INDOOR
  • AIR TEST FOR MOLD: ACCURACY
  • AIRBORNE MOLD COUNT NUMBER GUIDE
  • AIRBORNE PARTICLE ANALYSIS METHODS
  • ALLERGEN TESTS for BUILDINGS
  • ASBESTOS IDENTIFICATION IN BUILDINGS
  • BIOLOGICAL POLLUTANTS
  • CARPETING & INDOOR AIR QUALITY
  • CAT DANDER in BUILDINGS
  • CHEMICAL CONTAMINANTS in WATER
  • COMBUSTION GASES & PARTICLE HAZARDS
  • DIRECTORY of MOLD / ENVIRONMENTAL EXPERTS
  • EMF ELECTROMAGNETIC FIELDS & HUMAN EXPOSURE
  • FIBERGLASS IDENTIFICATION in the LAB
  • FORENSIC & IAQ FIELD IAQ EQUIPMENT SOP - home
  • FORENSIC & IAQ LAB MICROSCOPE TECHNIQUES
  • FORENSIC LAB TECHNICAL PROCEDURES
  • FORENSIC & IAQ LABORATORY SERVICES
  • FLOOR TILE ASBESTOS IDENTIFICATION
  • GAS DETECTION INSTRUMENTS
  • HOUSE DUST ANALYSIS
  • LIGHT, GUIDE to FORENSIC USE
  • LIGHT, UV BLACK LIGHT USES
  • METHANE GAS SOURCES
  • MICROSCOPE DIGITAL PHOTOGRAPHY
  • MICROSCOPE SLIDE PREPARATION
  • MICROSCOPE SLIDE PREP, PERMANENT MOUNT
  • MICROSCOPE TECHNIQUES for the LAB
  • MOLD TESTING METHOD VALIDITY
  • PAINT ANALYSIS, DIAGNOSTIC USES
  • PAINT FAILURE ANALYSIS LAB PHOTOS
  • PAINT FAILURE Case Photographs-SITE
  • PAINT FAILURE Case Photographs-LAB
  • PAINT LAB SAMPLE PREPARATION
  • PARTICLE SIZES & IAQ
  • SICK HOUSE IAQ QUESTIONNAIRE
  • SMELL PATCH TEST to Track Down Odors
  • THERMAL IMAGING, THERMOGRAPHY
  • THERMAL IMAGING MOLD SCANS
  • TOXIC GAS TEST PROCEDURES
  • VINYL Siding or PLASTIC Window ODORS
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How to calculate airborne mold or other airborne particle levels. This document describes proper procedures for evaluating mold and other aerobiological samples for purposes of identification of environmental sample contents and to help in assessing potential exposure of building occupants to levels of indoor particles, mold, allergens, and other materials. Included are procedures for proper particle counting from air samples, lab chemistry for sample preparation, and a directory of field sampling practices and methodology. References are also given for particle identification. This is Daniel Friedman's general aerobiology & forensic procedure. Other mold test laboratories & forensic laboratories follow similar procedures but details and policy may vary. For clarity, some topics are addressed in separate documents listed here.

Green links show where you are. © Copyright 2013 InspectAPedia.com, All Rights Reserved. Author Daniel Friedman.

How A Mold Lab or Forensic Lab Calculates Airborne Particle Concentration Levels

Example % of Trace Calculations for an Olympus CH-2 microscope #5F0837

Particle trace photo (C) Daniel FriedmanTo form an accurate understanding of the percentage of particle trace that has been examined in the microscope we need to know the physical area of the particle trace and the physical area encompassed by one field of view in the microscope for a given microscope objective (e.g. 40x or 100x objective).

[The actual magnification in the field of view is the product of the microscope objective magnification and the microscope eyepiece magnification. Typically the eyepiece magnification is 10x or 15x.

Typical microscope objective magnifications used for aerobiology, mold particles, pollen, and other small particle identification and counting are 40x, 60x, 100x or 120x. Indeed while counting is faster and easier at the lower magnifications of 40x or 60x, only by going to 100x oil immersion can we reliably see and count small particles in the 1-5u range, such as Penicillium or Aspergillus spores.

Air Sampling Equipment Trace Dimensions

Air test for mold, particle trace variation (C) Daniel FriedmanIf we are counting less than 100 percent of the particle trace, the dimensions of the trace width and length are required to complete airborne particle concentration calculations. While we give the standardized particle trace dimensions and the percentage represented by one pass across the trace at different magnifications (below), keep in mind that the true particle trace on an air sampler slide varies due to site conditions and other factors - as you can see in our example photo at left.

See LAB & FIELD IAQ EQUIPMENT SOP for a complete table of airborne particle trace dimensions for various air sampling devices.

Air-O-Cell @1000x: .173mm field width/14.4mm x 100 = 1.2% of trace per pass

Air-O-Cell @ 400x:.44mm/14.4mm x 100 = 3.05% of trace per pass

Burkard @ 1000x: .173mm/14.0mm x 100 = 1.24% of trace per pass

Burkard @ 400x: .44mm/14.0mm x 100 = 3.14% of trace per pass

Allergenco @ 1000x: .173mm/.145mm x 100 = 1.193% of trace per pass

Allergenco @ 400x: .44mm/.145mm x 100 = 3.03% of trace per pass (e.g. use .303 as divisor in formula below)

(WARNING: this data is calibrated for a particular microscope in our lab. This number must be calibrated to the microscope, and optics used for the examination of the trace).

A Detailed Example for Airborne Particle Concentration Counting Calculations using the Allergenco Time-Lapse Impaction Sampler

Stachybotrys spores (left) and structure (right)To compute the airborne concentration of any individual particle (or of all particles) in an air test sample we need to know the air sample volume (total liters), for which we need to know the air sampling device flow rate in liters per minute and the number of minutes the sampler was run. We also need to allow for cases in which we count less than 100 percent of the particle trace. The basic formula for calculating the airborne particle concentration in an air sample is

Particles/M3 = [ 1000 liters per cubic meter / liters of air sampled ] x [Raw particle count / % of particle trace examined]

1. Sample Volume in Liters = (Sampler run time in minutes x calibrated sampler flow rate in LPM)One M3 is 1000 cubic liters. So another version of the calculation could use

2. Sample Volume in M3 = [(Flow rate in LPM)/1000] x [run time in minutes]
(or if you prefer, 15 lpm=.015 M3/m )

To obtain the total volume of air that was processed during an air test for airborne particles we multiply the air flow rate of the sampler (e.g. 15 lpm) by the number of minutes the sampler was operated (e.g. 3 minutes).

E.g. if we ran our Burkard Personal Air Sampler for 7 minutes, knowing that this devices pulls air through its impaction sampler slit at 10 liters per minute, 10 x 7 = 70 liters of air in our sample.

3. Percentage of the total particle trace that was examined. As we explain below, very dense or overloaded samples may have so many particles as to preclude an accurate count of 100 % of the trace sample. If we are counting less than 100% of the sample we need to include that factor in our calculations.

Trace length counted = (Microscope field diam) x (# cross-width traverses)

4. Particles / M3 = (raw particle count / % portion of trace counted) x (1000L/M3 / sample volume in L)

Example 1 - Number of particles represented by a 10 minute air test using an Allergenco impaction sampler

if an Allergenco time-lapse impaction air sampler is run for 10 minutes, 150 L was sampled (10 minutes x 15 LPM)

For the Allergenco, 1 cross trace pass at 1000x = 1.193% of trace

For the Allergenco, 10 cross-trace passes = 11.93% of the trace, or .1193 of the trace.

Example: If Particle "x" was counted at 653 particles in 10 passes of the trace at 1000x.

Example: if 100% of the trace was counted, then the "raw count" will be divided by 1 (100%)

[653 particles / (.1193 of trace/pass )] x [1000 L in 1 M3 of air / 150 L in sample] = 34,490.6 particles/M3 of air in the sample. This number should be documented in the lab as 34,490.6 but in an interpretation may be described as 34,500 particles/M3 of air.

Airborne particle concentration is found to be 34,500 particles/M3 of air.

Example 2 - Airborne mold concentration represented by an 8 minute air test using a Burkard Personal Sampler

The Burkard PAS samples air at 10 lpm, so an 8-minute sample represents a test volume of (10 x 8) = 80 liters of air.

We counted 100% of the particle sample trace in this case, identifying the number of occurrences of each particle type found in the sample. Using just one of these particles, Alternaria sp., we found 7 Alternaria spores in the entire trace.

Particles / M3 = (raw particle count / % portion of trace counted) x (1000L/M3 / sample volume in L)

Alternaria mold spores / M3 = ( 7 / 1.00 ) x ( 1000L/M3 / 80L ) = 88 Alternaria mold spores / M3

An Alternative count procedure for Airborne Particle Concentrations

Measure the actual trace length. Divide the trace into equal segments by 4ths

Count 1 segment completely, selecting a representative segment after scanning lengthwise the whole trace. Multiply the actual count by the number of segments (4) to get the "trace total count. ["trace total count"] x [1000/sample volume] = particles/M3

General Counting Rules for Airborne Mold or Other Particle Concentrations in Air Tests & Samples

Interferences in Reaching the Objective of 100% Trace Counting - handling overloaded air particle samples

Air plenum sample (C) Daniel FriedmanFor low density particle traces it is easy and reasonable, as well as most accurate, to count 100% of the particle trace area. Where practical, in our lab we count 100% of the particle trace on the sampling slide or cassette.

Our photo (left) illustrates a reasonable-density air particle sample trace on a slide from one of our Burkard Personal Air Sampler devices. By examination using the naked eye the trace density appears not to be overloaded, though final decision on how to process this sample will be made after the initial examination in the microscope. This particle sample was collected inside the return air plenum of a heating & air conditioning air handler, using passive air sampling methods - we just placed the sampler in the space and ran it for a number of minutes, with minimum disturbance to the environment. And of course the equipment was OFF during the test.

But some airborne particle or dust vacuum particle traces are so dense with particles that an accurate particle count can be difficult, impractical, or even impossible. Our second Burkard PAS particle trace (below left) was collected in the same location as the first particle trace above, but we used our standard "aggressive" sampling method intended to dislodge local particles that might otherwise not appear in the test.

Particle trace from an air plenum (C) Daniel FriedmanWe rapped once with a flashlight on the side of the air handler's air plenum chamber. You can see that this approach made an enormous difference in the number of particles collected even though we ran the air sampling device for the same time interval as in the first test. This sample is probably overloaded.

For example, a very dense particle trace suggests that during the latter portion of the air sampling period, late-arriving particles may impact particles already on the trace medium surface rather than contacting the more sticky trace capture media. As a result, the particle bounce rate increases late in the air sampling period - such particles are lost and thus under-represented in the trace result.

For thick, occluded, dense particle traces too, the absolute number or raw particle count could be in the thousands, making accurate manual counting inaccurate or infeasible. For such dense particle traces our minimum objective is to count 25% of the trace. 20% may be used in the most difficult cases.

We first scan the entire particle trace at lower magnification, perhaps 400x, in order to note the presence or absence of anomalies such as voids in the trace capture media or thick clusters of particles or large particles that can substantially affect the accurate particle count number and that might be missed during a 25% random trace area count approach.

Particle or mold spore cluster or spore chain counting & reporting rules

Airborne particles San Miguel de Allende (C) Daniel FriedmanBelieve it or not some mold test labs count the four spores seen at left as "one" particle because they occur in a single cluster.

Similarly, and more important, some mold test labs count a chain of connected mold spores such as Aspergillus spores or Penicillium spores as "one" spore.

Inconsistencies in how labs count clusters and chains of particles can make a very large difference in the airborne particle concentration reported.

We count the individual spores in clusters or chains, and we use that number in our airborne particle concentration calculations.

But because the presence of Pen/Asp spores in chains in an indoor air sample screening for building mold contamination is very important, we also report the presence of spores in chains as a separate technical examination.

Airborne Particle Trace Count Stopping Rules

Airborne Particle Trace Count Stopping Rules may be considered. (when 200 particles/M3 are reached continue that pass across the particle trace to completion, record the total count, calculate the total based on % of trace read, and express it as a minimum with citation of stopping rule.)

IAQ Investigation and Airborne Mold or Particle Level Reporting requirements

See MOLD LAB REPORTS.

Also see ACCURACY vs PRECISION of MEASUREMENTS where we argue that measurements should be reported to include their percentage of error or a +/- figure to give a realistic understanding of the actual reliability of the data.

Indoor Air Quality Sampling Equipment Calibration Procedures

Also see the following forensic lab & microscopy equipment calibration and adjustment details in these individual lab equipment procedures notebook or policy documents, including the latest microscope calibration dates.

  • Procedures Microscopy Count Calibrate ™ directory including Trace calculations recap.doc (this file in non-html form)
  • Procedures Lab SOP Chemistry ™ directory Lab SOP - @LAB SOP.doc
  • Procedures Field ™ directory @SampMeth.doc (field method descriptions, comparisons) including bulksamp.htm (client-submitted tape sample procedure) and IAQMethods.htm (equipment lists) and see LAB & FIELD IAQ EQUIPMENT SOP for notes on air sampling equipment calibration.
  • Particle Identification ™ directory (disk library and reference texts in lab)

Microscope calibration:

Review microscope setup, Kohler illumination, objective centering, stage micrometer calibration of eyepiece reticule scale, and other preparation procedures in the lab notebook dedicated to each microscope. See LAB PROCEDURES MICROSCOPE TECHNIQUES for a discussion of microscope adjustment, setup, and calibration procedures. For each microscope (or other equipment) the lab maintains a procedures manual that gives these details as well as records of calibration and adjustment dates and results.

  • Olympus Microscope Operation
  • Polam Microscope Operation
  • Stereo Microscope Operation
  • Projecting Microscope Operation
  • Review objective/eyepiece field width measurement data at spread sheet dedicated to each microscope

  • Olympus-CH-2.xls
  • Polam Optics.xls

Lab Chemicals for Particle Sample Examination

review list of chemicals, uses, preparations in the Lab Procedures ™ Chemistry & Slide Preparation notebook. Review the MSDS for each lab chemical in the Lab Supplies MSDS notebook. Key files:

  • MICROSCOPE SLIDE PREPARATION
  • LAB PROCEDURES MICROSCOPE TECHNIQUES
  • TECHNICAL & LAB PROCEDURES
  • @LABChemicals.doc
  • @slide preparation.doc
  • Meltmount tips.doc - also at MICROSCOPE SLIDE PREP - PERMANENT MOUNTS
  • Other files in directory Procedures Lab SOP Chemistry

Procedures for Qualitative Building Surface Particle Sample Analysis or Vacuum Sample Analysis for Surfaces, Carpets, Soft Goods

Tape or vacuum cassette (tape, Air-o-Cell, or filter cassette) samples are prepared using lab SOP for each sample type. Qualitative analysis and characterization is described in each lab report. Quantitative analysis of surface tape samples is highly questionable since particle density across a building surface cannot be assumed to be uniform.

For Air-o-Cell or MCE filter cassettes, scan the entire trace at low magnification, 40x or 100x, for consistency and for unusual particle clusters to be considered in selecting areas for cross-scans.

For tape samples, the sample may be examined using the low-power stereo microscope to evaluate sample consistency and to select a sample tape segment most-likely to contain significant particles. A 1 cm segment is selected for analysis. If the tape appears to contain a variety of particles by texture, color, etc., multiple 1-cm segments may be required for analysis.

Scan across the trace beginning at one end of the selected area, to and from areas where no particle are visible while crossing the trace. See separate counting rules and stopping rules (if any stopping rules are to be applied), obtaining raw counts of significant or other particles of interest in each pass and totaling for the trace. See Particle Counts.xls for worksheets used for this purpose. The worksheet automates particles/M3 of air when the raw counts and volume of the sample are entered.

 


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Technical Reviewers & References

Related Topics, found near the top of this page suggest articles closely related to this one.

  • AIRBORNE PARTICLE ANALYSIS METHODS - Airborne Particle Calculations, How to calculate airborne particle concentrations for mold, IAQ, allergens, or other forensic particle studies - Mold Test Laboratory SOPs
  • MICROSCOPE SLIDE PREPARATION - Microscope slide preparation methods - mold pollen bioaerosols - for indoor air quality analysis - Mold Testing Laboratory SOP
  • LAB PROCEDURES MICROSCOPE TECHNIQUES - Microscope techniques and laboratory procedures for indoor air quality, toxic mold identification, & bioaerosol investigations
  • LAB & FIELD IAQ EQUIPMENT SOP - Forensic Microscopy, Particle Identification & IAQ Investigation Field Equipment Specifications, including physical properties (such as particle trace dimensions) and calibration data for air sampling and other test equipment
  • MICROSCOPE DIGITAL PHOTOGRAPHY - how to obtain sharp photographs of views through the microscope using a digital camera
  • [6] "Particulate Matter Sampling", ATPI 435: Atmospheric Sampling Course, U.S. EPA, retrieved 9/9/12, original source: http://www.epa.gov/apti/Materials/APTI%20435%20student/Student%20Manual/Chapter_4_noTOC-cover_MRpf.pdf [copy on file as Particulate_Matter_Sampling_USEPA.pdf.]
  • TECHNICAL & LAB PROCEDURES - Technical Procedure Articles on Testing for Mold and other Indoor Air Quality IAQ Contaminants
  • [8] For most-recent microscope optical measurements: field width data and calibration see: Olympus-CH-2.xls & Polam Optics.xls Calibration is repeated no less than when an objective is removed/replaced or other microscope optical or stage components are removed/replaced, or if a new technician is employed.
  • For most-recent calculations of % of trace represented by n cross-scans see: Particle Counts.xls
  • For automated calculations of particles or spores per cubic meter of air (spores/M3) from raw airborne particle counts see: @SporeCountWork.xls
  • For spore count worksheets see: sheet 2 of @SporeCountWork.xls

Books & Articles on Building & Environmental Inspection, Testing, Diagnosis, & Repair

  • Our recommended books about building & mechanical systems design, inspection, problem diagnosis, and repair, and about indoor environment and IAQ testing, diagnosis, and cleanup are at the InspectAPedia Bookstore. Also see our Book Reviews - InspectAPedia.
  • Home Reference Book - Carson DunlopThe Home Reference Book - the Encyclopedia of Homes, Carson Dunlop & Associates, Toronto, Ontario, 25th Ed., 2012, is a bound volume of more than 450 illustrated pages that assist home inspectors and home owners in the inspection and detection of problems on buildings. The text is intended as a reference guide to help building owners operate and maintain their home effectively. Field inspection worksheets are included at the back of the volume. Special Offer: For a 10% discount on any number of copies of the Home Reference Book purchased as a single order. Enter INSPECTAHRB in the order payment page "Promo/Redemption" space. InspectAPedia.com editor Daniel Friedman is a contributing author.

    Or choose the The Home Reference eBook for PCs, Macs, Kindle, iPad, iPhone, or Android Smart Phones. Special Offer: For a 5% discount on any number of copies of the Home Reference eBook purchased as a single order. Enter INSPECTAEHRB in the order payment page "Promo/Redemption" space.

  • GO TO Carson Dunlop's Home Study Course Information - How to Become a Home Inspector: Carson Dunlop's nationally recognized Home Study Course, selected by ASHI the American Society of Home Inspectors and other professionals and associations. This website author is a contributor to this course.
  • GO TO Carson Dunlop's Home Study Course Information - How to Become a Home Inspector: Carson Dunlop's nationally recognized Home Study Course, selected by ASHI the American Society of Home Inspectors and other professionals and associations. This website author is a contributor to this course.
  • GO TO Carson Dunlop's Home Study Course Information - How to Become a Home Inspector: Carson Dunlop's nationally recognized Home Study Course, selected by ASHI the American Society of Home Inspectors and other professionals and associations. This website author is a contributor to this course.
    Building inspection education & report writing systems from Carson, Dunlop & Associates Ltd
  • Environmental Health & Investigation Bibliography - our technical library on indoor air quality inspection, testing, laboratory procedures, forensic microscopy, etc.
  • Adkins and Adkins Dictionary of Roman Religion discusses Robigus, the Roman god of crop protection and the legendary progenitor of wheat rust fungus.
  • Kansas State University, department of plant pathology, extension plant pathology web page on wheat rust fungus: see http://www.oznet.ksu.edu/path-ext/factSheets/Wheat/Wheat%20Leaf%20Rust.asp
  • "A Brief Guide to Mold, Moisture, and Your Home", U.S. Environmental Protection Agency US EPA - includes basic advice for building owners, occupants, and mold cleanup operations. See http://www.epa.gov/mold/moldguide.htm
  • US EPA - Mold Remediation in Schools and Commercial Building [Copy on file at /sickhouse/EPA_Mold_Remediation_in_Schools.pdf ] - US EPA
  • US EPA - Una Breva Guia a Moho - Hongo [Copy on file as /sickhouse/EPA_Moho_Guia_sp.pdf - en Espanol

Allergies, Allergens, Allergy Testing in Buildings - References & Products

  • Air Conditioning System Blower Fans & Filters Cascading for Optimum Indoor Air Quality
  • Allergen Tests in Buildings advice about how to test, what to look for, in evaluating the level of dog, cat, or other animal allergens in a building
  • "IgG Food Allergy Testing by ELISA/EIA, What do they really tell us?" Sheryl B. Miller, MT (ASCP), PhD, Clinical Laboratory Director, Bastyr University Natural Health Clinic - ELISA testing accuracy: Here is an example of Miller's critique of ELISA http://www.betterhealthusa.com/public/282.cfm - Townsend Letter for Doctors and Patients
    The critique included in that article raises compelling questions about IgG testing assays, which prompts our interest in actually screening for the presence of high levels of particles that could carry allergens - dog dander or cat dander in the case at hand.
    http://www.tldp.com/issue/174/IgG%20Food%20Allergy.html contains similar criticism in another venue but interestingly by the same author, Sheryl Miller. Sheryl Miller, MT (ASCP), PhD, is an Immunologist and Associate Professor of Basic and Medical Sciences at Bastyr University in Bothell, Washington. She is also the Laboratory Director of the Bastyr Natural Health Clinic Laboratory.
  • Allergens: Testing for the level of exposure to animal allergens is discussed at http://www.animalhealthchannel.com/animalallergy/diagnosis.shtml (lab animal exposure study is interesting because it involves a higher exposure level in some cases
  • Allergens: WebMD discusses allergy tests for humans at webmd.com/allergies/allergy-tests
  • Animal Allergens: Dog, Cat, and Other Animal Dander - Cleanup & Prevention Information for Asthmatics and regarding Indoor Air Quality.
  • Atlas of Mold Related Illness Symptoms & Complaints - long list of both documented, studied mold related illness, and complaints ascribed to mold contamination or allergens in buildings
  • Cat Dander: how to inspect and test a building for past or current presence of cats, cat hair, cat dander, and cat allergens
  • Clinical Atlas of Mold Toxicity - An Online Description of Toxic, Pathogenic, Allergenic Fungi, Fungal Diseases
  • Fiberglass Insulation Contains Mold© 2005 comments about a field study in process, & more about health hazards from fiberglass insulation
  • Humidity: What indoor humidity should we maintain in order to avoid a mold problem?
  • Mold Action Guide detailed guide on finding, removing, and preventing indoor mold contamination
  • Odors, Odor Detection, Smells, & Gases how to find and identify sources of noxious or toxic odors and gases
  • Other environmental risks, Our much longer list: Asbestos, carbon monoxide, electromagnetic fields, etc.
  • Ozone: The Use of Ozone Indoors for Control of Odors and Mold Removal in Buildings: A Summary of Hazards and False Claims.
  • Pollen Allergens: identification, plant pollen and indoor air quality
  • Products to Reduce Mold & Allergy Problems to reduce indoor mold or allergen levels: air cleaners, air purifiers, dust mite covers, vacuum cleaners, crawl space vents
  • Recognizing Allergens: What various indoor allergens look like - identification photos to help identify pollen, dust mites, animal dander, toxic or allergenic mold - Common Mold and other Allergens, Irritants, Remedies & Advice
  • Rodent control issues, including dander, fecal, and urine contamination of Buildings and Building insulation are discussed at our
  • Sewage and Septic backup contamination in Buildings: inspection, testing, remediation, & references to expert sources
  • Action Guide: What to do about mold, mildew, and other indoor allergens
  • ...

Mold Contamination Testing, Cleanup, Prevention: references & products

  • GO TO the MOLD and INDOOR ENVIRONMENT INFORMATION CENTER for in-depth advice on avoiding testing for or cleaning up mold and other indoor environmental hazards, odors, gases, contaminantsThe Mold Information Center: What to Do About Mold in Buildings, When and How to Inspect for Mold, Clean Up Mold, or Avoid Mold Problems
  • Aerobiology, Building Science, Microscopy, & Laboratory References, an extensive technical bibliography
  • Allergens: what they look like in buildings
  • Associations: Sick House, Sick Building, SBS - Air Quality, Government, Private Associations and Information Resources
  • Atlas of Clinical Fungi, 2nd Ed., GS deHoog, J Guarro, J Gene, & MJ Figueras, Centraalbureau voor Schimmelcultures, Universitat Rovira I Virgili, 2000, ISBN 90-70351-43-9 (you can buy this book at Amazon) - The Atlas of Clinical Fungi is also available on CD ROM
  • Atlas of Mold Related Illness: Index of Symptoms and health, physical, neurological, psychological, and other complaint which people suspect may be mold or building-related.
  • Atlas of Indoor Mold, Online Clinical Mold Atlas, Toxins, Pathogens, Allergens and Other Indoor Particles - Medical Health Effects of Mold (separate online document)
  • Black Mold that is Harmless Photos of recognizable, usually harmless black mold on wood, bluestain, ceratocystis, ophistoma
  • Building Floods: quick steps after a building flood or plumbing leak can prevent costly mold contamination
  • Classes of Mold: what types of cosmetic, allergenic, or toxic mold are a problem? Can mold be cleaned-up successfully?
  • Clinical Mold References - Detailed bibliography of mold reference texts
  • "A Brief Guide to Mold, Moisture, and Your Home", U.S. Environmental Protection Agency US EPA - includes basic advice for building owners, occupants, and mold cleanup operations. See http://www.epa.gov/mold/moldguide.htm
  • "Disease Prevention Program for Certain Vegetable Crops," David B. Langston, Jr., Extension Plant Pathologist - Vegetables, University of Georgia (PDF document) original source: www.reeis.usda.gov/web/crisprojectpages/209797.html
  • "Disease Prevention in Home Vegetable Gardens," Patricia Donald, Department of Plant Microbiology and Pathology, Lewis Jett
    Department of Horticulture, University of Missouri Extension - extension.missouri.edu/publications/DisplayPub.aspx?P=G6202
  • "Management of Powdery Mildew, Leveillula taurica, in Greenhouse Peppers," Ministry of Agriculture and Lands, British Columbia - Original source: www.agf.gov.bc.ca/cropprot/peppermildew.htm
  • Environmental Health & Investigation Bibliography - our own technical library on indoor air quality inspection, testing, laboratory procedures, forensic microscopy, etc.
  • Fiberglass: Mold in Fiberglass Insulation© 2005 comments about a field study in process, & more about health hazards from fiberglass insulation - DJF
  • Fifth Kingdom, Bryce Kendrick, ISBN13: 9781585100224, is available from the InspectAPedia online bookstore - we recommend the CD-ROM version of this book. This 3rd/edition is a compact but comprehensive encyclopedia of all things mycological. Every aspect of the fungi, from aflatoxin to zppspores, with an accessible blend of verve and wit. The 24 chapters are filled with up-to-date information of classification, yeast, lichens, spore dispersal, allergies, ecology, genetics, plant pathology, predatory fungi, biological control, mutualistic symbioses with animals and plants, fungi as food, food spoilage and mycotoxins.
  • Fungi, Identifying Filamentous, A Clinical Laboratory Handbook, Guy St-Germain, Richard Summerbell, Star Publishing, 1996, ISBN 0-89863-177-7 (English) (buy at Amazon)
  • Looking for Mold Procedure: what mold is often found where in buildings - simple technical presentation
  • Meruliporia: the house eating fungus or "poria"
  • Mold Action Guide: Step-by-Step Instructions, What to do about mold, mildew, and other indoor allergens
  • MOLD APPEARANCE - WHAT MOLD LOOKS LIKE Photos of what mold looks like in buildings
  • MOLD APPEARANCE - STUFF THAT IS NOT MOLD Photos of NOT-mold material that is sometimes mistaken for mold
  • MOLD ATLAS & PARTICLES INDEX, Pathogens, Allergens and Other Indoor Particles - Medical Health Effects of Mold (separate online document)
  • MOLD BY MICROSCOPE Mold under the microscope - photo identification of the most common indoor molds found in buildings
  • Mold FAQs Answers to Most Questions about Indoor Mold, Mold Related Illness, Mold Cleanup, Mold Prevention
  • US EPA: Mold Remediation in Schools and Commercial Building [Copy on file at /sickhouse/EPA_Mold_Remediation_in_Schools.pdf ] - US EPA
  • Mold spores in the Home - a Photo ID Library for detection and identification of mold allergens
  • Mold Test Kits - How to Collect and Send Your Own Mold Sample to our mold testing lab or to any mold lab you wish
  • Most Common Indoor Molds Found in Buildings, A Table of
  • Mycology, Fundamentals of Diagnostic, Fran Fisher, Norma B. Cook, W.B. Saunders Co. 1998, ISBN 0-7216-5006-6 (buy this book at Amazon)
  • Ozone Warnings - Use of Ozone as a "mold" remedy is ineffective and may be dangerous.
  • Rot concerns in buildings-some building mold such as Meruliporia incrassata "Poria" risks serious rot and hidden structural damage
  • US EPA: Una Breva Guia a Moho - Hongo [Copy on file as /sickhouse/EPA_Moho_Guia_sp.pdf - en Espanol

OTHER IAQ ISSUES: How To Find and Address Other Indoor Air or Indoor Environment Contaminants Besides Mold

Mold or allergens may not be the only or even the main indoor environmental contaminant. Don't let media attention to mold cause so much enviro-scare fear that other, possibly more urgent hazards go un-addressed.

  • Fiberglass building insulation and HVAC duct work insulation hazards
  • Sewage and Septic backup contamination in buildings: inspection, testing, remediation, & references to expert sources
  • Other environmental risks: Asbestos, carbon monoxide, electromagnetic fields, environmental illness, fiberglass, MCS - multiple chemical sensitivity, toxic gases, etc
  • Indoor Gas Sampling Plan for Residential Buildings lists a number of toxic indoor gases which we test for, depending on the building complaint and building conditions
  • ...

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