InspectAPedia.com InspectAPedia®
Google
InspectAPedia
 

Free Encyclopedia of Building & Environmental Inspection, Testing, Diagnosis, Repair

Ask a Question or Search InspectAPedia

  • HOME
  • AIR CONDITIONING
  • DAMAGE ASSESSMENT
  • ELECTRICAL
  • EXTERIORS
  • HEATING
  • HOME INSPECTION
  • INTERIORS
  • PLUMBING
  • ROOFING
  • SEPTIC SYSTEMS
  • STRUCTURE
  • WATER SUPPLY
  • ENERGY SAVINGS
  • ENVIRONMENT
  • INDOOR AIR IAQ
  • INSULATION
  • MOLD INSPECT TEST REMOVE
  • NOISE
  • ODORS
  • SOLAR ENERGY
  • VENTILATION
  • EXPERTS DIRECTORY
  • CONTACT US




Mobile View
MOLD: A COMPLETE GUIDE to TEST CLEAN PREVENT

AIR CLEANER PURIFIER TYPES
AIR FILTERS for HVAC SYSTEMS
AIR POLLUTANTS, COMMON INDOOR
AIR QUALITY IMPROVEMENT STRATEGIES
ALLERGEN TESTS for BUILDINGS
ANIMAL ALLERGENS / PET DANDER
ANIMAL ODORS IN BUILDINGSS
ATTORNEYS and EXPERT WITNESSES

BASEMENT MOLD
BATHROOM MOLD
BIBLIOGAPHY ENVIRONMENTAL HEALTH, MOLD, IAQ
BIOLOGICAL POLLUTANTS

CAR MOLD CONTAMINATION
CARPET DUST IDENTIFICATION
CARPET MOLD CONTAMINATION
CAT DANDER in BUILDINGS
COMBUSTION GASES & PARTICLE HAZARDS
CONDENSATION or SWEATING PIPES, TANKS
CPSC Indoor Air Pollution Book Online Copy

DUCT SYSTEM & DUCT DEFECTS

EMERGENCY RESPONSE, IAQ, GAS, MOLD

FIBERGLASS HAZARDS
FLOOD DAMAGE ASSESSMENT, SAFETY & CLEANUP
FLOODS IN BUILDINGS-mold
FORMALDEHYDE HAZARDS

GAS EXPOSURE EFFECTS, TOXIC

HUMIDITY CONTROL & TARGETS INDOORS
HOUSE DUST ANALYSIS

INDOOR AIR QUALITY IMPROVEMENT GUIDE
INSULATION MOLD

MILDEW REMOVAL & PREVENTION
MOISTURE CONTROL in BUILDINGS
MOLD GROWTH on SURFACES, TABLE OF
MYCOPHOBIA, STAINS MISTAKEN for MOLD

NOISE / SOUND DIAGNOSIS & CURE

ODORS GASES SMELLS, DIAGNOSIS & CURES
OZONE HAZARDS
OZONE for MOLD OR ODORS

RADON HAZARD TESTS & MITIGATION

SAFETY HAZARDS GUIDE
SICK HOUSE IAQ QUESTIONNAIRE
SMELL PATCH TEST to Track Down Odors
STAIN DIAGNOSIS on BUILDING INTERIORS

VENTILATION in BUILDINGS

More Information

Alternaria mold spores and hyphae from an indoor surface (C) Daniel FriedmanUsing Vacuum Cassettes or Spore Traps to Collect Mold or Particle Dust Samples from Building Insulation
     

  • How to test building insulation for mold contamination
    • Guide to use of vacuum cassettes for mold screening
    • Find mold hidden in building insulation that looks clean
    • Photographs of mold contaminated fiberglass insulation
  • MOLD CULTURE TEST KIT VALIDITY - separate article
  • SWAB & PCR SAMPLING & TESTS for MOLD - separate article
  • TAPE & BULK SAMPLING & TESTS for MOLD - separate article
  • VACUUM CASSETTE FILTER SAMPLE TESTS for DUST / MOLD - separate article
    • VACUUMING BUILDING CAVITIES - separate article
    • VACUUMING BUILDING INSULATION
    • VACUUM SAMPLING EQUIPMENT, DIY - separate article
  • Questions & answers about vacuuming building insulation to screen for mold or other hazardous particle contamination.
  • References

Click to Show or Hide Related Topics

  • ACCURACY OF VARIOUS MOLD TEST METHODS - home
  • ACCURACY vs PRECISION of MEASUREMENTS
  • AIR TEST SAMPLING CASSETTE STUDY
  • AIRBORNE MOLD COUNT NUMBER GUIDE
  • AIRBORNE MOLD COUNT VALIDITY
  • AIRBORNE PARTICLE ANALYSIS METHODS
  • CONCENTRATION BURSTS of Mold Spores
  • CULTURE PLATE Test Errors, Mold
  • DUST SAMPLING PROCEDURE
  • FALSE NEGATIVEW Results in Mold Tests
  • HIDDEN MOLD, HOW TO FIND
  • INDOOR vs OUTDOOR Airborne Spore Counts
  • LAB & FIELD IAQ EQUIPMENT SOP
  • LAB PROCEDURES MICROSCOPE TECHNIQUES
  • LAB PROCEDURES, TECHNICAL
  • MOLD LEVEL IN AIR, VALIDITY
  • MOLD STANDARDS
  • MOLD TESTING METHOD VALIDITY
  • PARTICLE & MOLD LEVELS in DUCTWORK
  • SLIDE PREPARATION, MICROSCOPE
  • VALIDITY of MOLD TESTING METHODS
  • VARIATION IN AIRBORNE Particle Levels, Causes of
  • VARIATION IN AIRBORNE Particle Levels vs Sampler Height
  • VARIATION IN AIRBORNE PARTICLE COUNTS, Extent of
InspectAPedia tolerates no conflicts of interest. We have no relationship with advertisers, products, or services discussed at this website.

Vacuum tests on building insulation to screen for mold or other particles: this article explains the advantages and shortcomings of using vacuum cassettes or spore traps to collect mold test samples (or other dust or particle samples) from building insulation where mold, insect, or other contamination is suspected. Our photo (page top) shows what looked like clean fiberglass insulation located over a basement that had been subject to chronic flooding and ultimately, mold contamination that was visible on other basement surfaces. Vacuum tests confirmed that the insulation contained high levels of Penicillium/Aspergillus mold spores and was acting as a mold reservoir in the building.

Green links show where you are. © Copyright 2013 InspectAPedia.com, All Rights Reserved. Author Daniel Friedman.

Usefulness of Vacuuming exposed Building Insulation for Mold Contamination

Photograph of crawl space insulation which testing found to be mold contaminated.15th Annual North Carolina/South Carolina
Environmental Information Association Technical Conference
Myrtle Beach, SC
Daniel Friedman 23 September 2005, Updated 4/14/2009

At many investigations we have found a large hidden mold reservoir in building insulation, particularly fiberglass insulation in attics under roof leaks over drywall, and in crawl spaces which have been damp or wet. We have also found very moldy fiberglass in basement ceilings after moldy surfaces and debris have been removed (such insulation should have been removed during the remediation).

Our photo (left) shows insulation that did not display visible mold but that our vacuum test found to be very mold-contaminated (see our lab photo below). (The dark stains on this fiberglass insulation were not mold but house dust deposited from air movement - see THERMAL TRACKING & THERMAL BRIDGING for details.)

Our method is to agitate the target insulation (simply poke it with your flashlight or a ruler), followed by holding our vacuum cassette an inch or two away from the insulation surface. This reliably picks up particles from the insulation without overloading the sample with fiberglass. (A baseline comparison sample collected in nearby building air before any such agitating sampling is also needed.) This method has been remarkably successful in finding and allowing the removal of several "mystery" problems in buildings where severe mold-related complaints were heard.

Photograph of mold spores of Aspergillus sp. found in crawl space fiberglass insulation.Any of several types of vacuum cassettes are used to collect dust from a surface. We use Air-o-Cel ™ cassettes and MCE filter cassettes. We am experimenting with vacuum cassettes loaded with high-adhesive tape.

The contents of the mold test vacuum cassette may be examined by light microscope or may be used for preparation of cultures. One special (and costly) cassette method collects dual samples permitting both direct examination and culturing.

Our lab photo (left) shows insulation that did not display visible mold to the naked eye when examined in place in the building (photo above) but that our vacuum test found to be very mold-contaminated.

This method may be used for both qualitative and quantitative analysis, depending on collection method details. It is best suited for sampling dust from surfaces and from soft goods such as carpeting or upholstered furniture.

It's strength is its use in examining multiple hard surfaces with relatively low levels of debris (avoiding sample overload) or individual soft surfaces where tape may not collect particles imbedded in the surface, and in collecting dust from multiple locations in a single cassette as a building dust scan for mold.

Shortcomings of vacuuming insulation for mold

We love this method, but one must take care not to overload the sample.

If insulation is not exposed for testing one needs to make a sufficiently large opening to agitate and then vacuum the insulation - we use a 4" square opening and take care to avoid vacuuming up simply a collection of drywall dust.

See  Mold in Fiberglass Insulation for a detailed discussion of hidden mold contamination in building insulation. Also see Fiberglass Detection in Building Air.

In this article series discuss the validity of nearly all of the popular mold testing methods currently in use, pointing out the strengths and weakness of each approach to mold sampling in the indoor environment, beginning with air sampling for airborne mold levels indoors. Because mold test validity and mold test accuracy are often confused, readers should also see ACCURACY OF VARIOUS MOLD TEST METHODS. People who need to conduct mold inspection and testing indoors should see MOLD TEST PROCEDURES and TECHNICAL & LAB PROCEDURES.

Our MOLD INFORMATION CENTER includes more broad discussions of the overall approach to building investigation, as do many expert references cited at that web. For a more comprehensive collection information about mold test methods see INDOOR AIR QUALITY METHODS COMPARED. For more on "mold classes" (Cosmetic mold vs. allergenic mold vs. toxic or pathogenic mold) see MOLD CLASSES, HAZARD LEVELS and more references such as a Mold Action Guide are at the end of this document.


Frequently Asked Questions (FAQs) about using vacuuming methods to test building insulation for contamination by mold, insects, allergens or other particles

...

Ask a Question or Search InspectApedia

Use the search box below to ask a question or to search the InspectApedia.com website.

Ask a Question or Enter Search Terms in the InspectApedia search box just below.

Technical Reviewers & References

Related Topics, found near the top of this page suggest articles closely related to this one.

  • Kansas State University, department of plant pathology, extension plant pathology web page on wheat rust fungus: see http://www.oznet.ksu.edu/path-ext/factSheets/Wheat/Wheat%20Leaf%20Rust.asp
  • "A Brief Guide to Mold, Moisture, and Your Home", U.S. Environmental Protection Agency US EPA - includes basic advice for building owners, occupants, and mold cleanup operations. See http://www.epa.gov/mold/moldguide.htm
  • US EPA - Mold Remediation in Schools and Commercial Building [Copy on file at /sickhouse/EPA_Mold_Remediation_in_Schools.pdf ] - US EPA
  • US EPA - Una Breva Guia a Moho - Hongo [Copy on file as /sickhouse/EPA_Moho_Guia_sp.pdf - en Espanol
  • "IgG Food Allergy Testing by ELISA/EIA, What do they really tell us?" Sheryl B. Miller, MT (ASCP), PhD, Clinical Laboratory Director, Bastyr University Natural Health Clinic - ELISA testing accuracy: Here is an example of Miller's critique of ELISA http://www.betterhealthusa.com/public/282.cfm - Townsend Letter for Doctors and Patients
    The critique included in that article raises compelling questions about IgG testing assays, which prompts our interest in actually screening for the presence of high levels of particles that could carry allergens - dog dander or cat dander in the case at hand.
    http://www.tldp.com/issue/174/IgG%20Food%20Allergy.html contains similar criticism in another venue but interestingly by the same author, Sheryl Miller. Sheryl Miller, MT (ASCP), PhD, is an Immunologist and Associate Professor of Basic and Medical Sciences at Bastyr University in Bothell, Washington. She is also the Laboratory Director of the Bastyr Natural Health Clinic Laboratory.
  • Allergens: Testing for the level of exposure to animal allergens is discussed at http://www.animalhealthchannel.com/animalallergy/diagnosis.shtml (lab animal exposure study is interesting because it involves a higher exposure level in some cases
  • Allergens: WebMD discusses allergy tests for humans at webmd.com/allergies/allergy-tests
  • Atlas of Clinical Fungi, 2nd Ed., GS deHoog, J Guarro, J Gene, & MJ Figueras, Centraalbureau voor Schimmelcultures, Universitat Rovira I Virgili, 2000, ISBN 90-70351-43-9 (you can buy this book at Amazon) - The Atlas of Clinical Fungi is also available on CD ROM
  • "A Brief Guide to Mold, Moisture, and Your Home", U.S. Environmental Protection Agency US EPA - includes basic advice for building owners, occupants, and mold cleanup operations. See http://www.epa.gov/mold/moldguide.htm
  • "Disease Prevention Program for Certain Vegetable Crops," David B. Langston, Jr., Extension Plant Pathologist - Vegetables, University of Georgia (PDF document) original source: www.reeis.usda.gov/web/crisprojectpages/209797.html
  • "Disease Prevention in Home Vegetable Gardens," Patricia Donald, Department of Plant Microbiology and Pathology, Lewis Jett
    Department of Horticulture, University of Missouri Extension - extension.missouri.edu/publications/DisplayPub.aspx?P=G6202
  • "Management of Powdery Mildew, Leveillula taurica, in Greenhouse Peppers," Ministry of Agriculture and Lands, British Columbia - Original source: www.agf.gov.bc.ca/cropprot/peppermildew.htm
  • Environmental Health & Investigation Bibliography - our own technical library on indoor air quality inspection, testing, laboratory procedures, forensic microscopy, etc.
  • Fiberglass: Mold in Fiberglass Insulation© 2005 comments about a field study in process, & more about health hazards from fiberglass insulation - DJF
  • Fifth Kingdom, Bryce Kendrick, ISBN13: 9781585100224, is available from the InspectAPedia online bookstore - we recommend the CD-ROM version of this book. This 3rd/edition is a compact but comprehensive encyclopedia of all things mycological. Every aspect of the fungi, from aflatoxin to zppspores, with an accessible blend of verve and wit. The 24 chapters are filled with up-to-date information of classification, yeast, lichens, spore dispersal, allergies, ecology, genetics, plant pathology, predatory fungi, biological control, mutualistic symbioses with animals and plants, fungi as food, food spoilage and mycotoxins.
  • Fungi, Identifying Filamentous, A Clinical Laboratory Handbook, Guy St-Germain, Richard Summerbell, Star Publishing, 1996, ISBN 0-89863-177-7 (English) (buy at Amazon)
  • US EPA: Mold Remediation in Schools and Commercial Building [Copy on file at /sickhouse/EPA_Mold_Remediation_in_Schools.pdf ] - US EPA
  • Mycology, Fundamentals of Diagnostic, Fran Fisher, Norma B. Cook, W.B. Saunders Co. 1998, ISBN 0-7216-5006-6 (buy this book at Amazon)
  • US EPA: Una Breva Guia a Moho - Hongo [Copy on file as /sickhouse/EPA_Moho_Guia_sp.pdf - en Espanol
  • Mark Cramer Inspection Services Mark Cramer, Tampa Florida, Mr. Cramer is a past president of ASHI, the American Society of Home Inspectors and is a Florida home inspector and home inspection educator. (727) 595-4211 mark@BestTampaInspector.com

Books & Articles on Building & Environmental Inspection, Testing, Diagnosis, & Repair

  • Our recommended books about building & mechanical systems design, inspection, problem diagnosis, and repair, and about indoor environment and IAQ testing, diagnosis, and cleanup are at the InspectAPedia Bookstore. Also see our Book Reviews - InspectAPedia.
  • Home Reference Book - Carson DunlopThe Home Reference Book - the Encyclopedia of Homes, Carson Dunlop & Associates, Toronto, Ontario, 25th Ed., 2012, is a bound volume of more than 450 illustrated pages that assist home inspectors and home owners in the inspection and detection of problems on buildings. The text is intended as a reference guide to help building owners operate and maintain their home effectively. Field inspection worksheets are included at the back of the volume. Special Offer: For a 10% discount on any number of copies of the Home Reference Book purchased as a single order. Enter INSPECTAHRB in the order payment page "Promo/Redemption" space. InspectAPedia.com editor Daniel Friedman is a contributing author.

    Or choose the The Home Reference eBook for PCs, Macs, Kindle, iPad, iPhone, or Android Smart Phones. Special Offer: For a 5% discount on any number of copies of the Home Reference eBook purchased as a single order. Enter INSPECTAEHRB in the order payment page "Promo/Redemption" space.

  • GO TO Carson Dunlop's Home Study Course Information - How to Become a Home Inspector: Carson Dunlop's nationally recognized Home Study Course, selected by ASHI the American Society of Home Inspectors and other professionals and associations. This website author is a contributor to this course.
  • GO TO Carson Dunlop's Home Study Course Information - How to Become a Home Inspector: Carson Dunlop's nationally recognized Home Study Course, selected by ASHI the American Society of Home Inspectors and other professionals and associations. This website author is a contributor to this course.
  • GO TO Carson Dunlop's Home Study Course Information - How to Become a Home Inspector: Carson Dunlop's nationally recognized Home Study Course, selected by ASHI the American Society of Home Inspectors and other professionals and associations. This website author is a contributor to this course.
    Building inspection education & report writing systems from Carson, Dunlop & Associates Ltd
  • Environmental Health & Investigation Bibliography - our technical library on indoor air quality inspection, testing, laboratory procedures, forensic microscopy, etc.
  • ...

HOME ABOUT CONTACT COPYING DESCRIPTION POLICIES PRINTING PRIVACY © 2013 Copyright InspectAPedia.com