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MOLD: A COMPLETE GUIDE to TEST CLEAN PREVENT

AIR CLEANER PURIFIER TYPES
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BASEMENT MOLD
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BIBLIOGAPHY ENVIRONMENTAL HEALTH, MOLD, IAQ
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CPSC Indoor Air Pollution Book Online Copy

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FLOODS IN BUILDINGS-mold
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GAS EXPOSURE EFFECTS, TOXIC

HUMIDITY CONTROL & TARGETS INDOORS
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More Information

Wall vacuum test (C) Daniel FriedmanVacuuming Building Cavities to Screen for Hidden Mold: Guide to Good Practices
     

  • Field testing vacuum tests of building cavities to check for mold contamination
  • Does vacuuming wall or ceiling cavities for mold produce reliable results?
  • MOLD CULTURE TEST KIT VALIDITY - separate article
  • SWAB & PCR SAMPLING & TESTS for MOLD - separate article
  • TAPE & BULK SAMPLING & TESTS for MOLD - separate article
  • VACUUM CASSETTE FILTER SAMPLE TESTS for DUST / MOLD - separate article
    • VACUUMING BUILDING CAVITIES
    • VACUUMING BUILDING INSULATION - separate article
    • VACUUM SAMPLING EQUIPMENT, DIY - separate article
  • Questions & answers about vacuuming building cavities to screen for dust or mold hazards
  • References

Click to Show or Hide Related Topics

  • ACCURACY OF VARIOUS MOLD TEST METHODS - home
  • ACCURACY vs PRECISION of MEASUREMENTS
  • AIR TEST SAMPLING CASSETTE STUDY
  • AIRBORNE MOLD COUNT NUMBER GUIDE
  • AIRBORNE MOLD COUNT VALIDITY
  • AIRBORNE PARTICLE ANALYSIS METHODS
  • CONCENTRATION BURSTS of Mold Spores
  • CULTURE PLATE Test Errors, Mold
  • DUST SAMPLING PROCEDURE
  • FALSE NEGATIVEW Results in Mold Tests
  • FORENSIC & IAQ FIELD IAQ EQUIPMENT SOP - home
  • FORENSIC & IAQ LAB MICROSCOPE TECHNIQUES
  • FORENSIC LAB TECHNICAL PROCEDURES
  • HIDDEN MOLD, HOW TO FIND
  • INDOOR vs OUTDOOR Airborne Spore Counts
  • MOLD LEVEL IN AIR, VALIDITY
  • MOLD STANDARDS
  • MOLD TESTING METHOD VALIDITY
  • PARTICLE & MOLD LEVELS in DUCTWORK
  • SLIDE PREPARATION, MICROSCOPE
  • VALIDITY of MOLD TESTING METHODS
  • VARIATION IN AIRBORNE Particle Levels, Causes of
  • VARIATION IN AIRBORNE Particle Levels vs Sampler Height
  • VARIATION IN AIRBORNE PARTICLE COUNTS, Extent of
InspectAPedia tolerates no conflicts of interest. We have no relationship with advertisers, products, or services discussed at this website.

Guide to vacuuming building cavities to screen for particles, dust, or mold: this article explains the advantages and shortcomings of using vacuum cassettes or spore traps to collect mold test samples (or other dust or particle samples) from building wall and ceiling cavities. In this article series discuss the validity of nearly all of the popular mold testing methods currently in use, pointing out the strengths and weakness of each approach to mold sampling in the indoor environment, beginning with air sampling for airborne mold levels indoors. Because mold test validity and mold test accuracy are often confused, readers should also see ACCURACY OF VARIOUS MOLD TEST METHODS. People who need to conduct mold inspection and testing indoors should see MOLD TEST PROCEDURES and TECHNICAL & LAB PROCEDURES.

Green links show where you are. © Copyright 2013 InspectAPedia.com, All Rights Reserved. Author Daniel Friedman.

Validity/Accuracy of Vacuuming Building Cavities for a Hidden Mold Screen

Wall vacuum test (C) Daniel Friedman15th Annual North Carolina/South Carolina
Environmental Information Association Technical Conference
Myrtle Beach, SC
Daniel Friedman 23 September 2005, Updated 4/14/2009

Vacuuming building cavities is a popular screening practice to look for mold reservoirs. The investigator is trying to explore wall cavities while doing minimal or no visible damage.

A wall-check™ system has been marketed for this purpose and several manufacturers have coped the basic idea: a receiving Air-o-Cel™ or MCE filter cassette is attached at its inlet side to a tube which is inserted as a probe into a wall cavity, permitting creation of only a small hole. The outlet or pump side of the test device is connected to a pump and operated, typically at 15 lpm. Where we have tested this method we have experimented with both passive collection (what most investigators use) or aggressive collection (banging on the wall/ceiling at various points to attempt to dislodge and stir particles).

Short duration samples, 1-2 minutes using an Air-o-Cell ™ permit a comparatively large number of samples to be collected in a reasonably short interval. Long duration samples, perhaps for up to two hours, are collected using an MCE filter cassette.

Our MOLD INFORMATION CENTER includes more broad discussions of the overall approach to building investigation, as do many expert references cited at that web. For a more comprehensive collection information about mold test methods see INDOOR AIR QUALITY METHODS COMPARED. For more on "mold classes" (Cosmetic mold vs. allergenic mold vs. toxic or pathogenic mold) see MOLD CLASSES, HAZARD LEVELS

Wall Cavity Vacuuming - Found Ineffective

Working with Louis Relle, a Louisiana mold remediation expert on a New Orleans Building which was to be demolished we collected wall-check samples from every suspect Building cavity.

When the building interior surfaces were demolished we then performed a visual inspection and collected bulk surface samples using tape. The wall check samples were completely unable to detect large and significant mold contamination in the cavities of this building.

We postulate that even with mechanical agitation (banging on the wall during wall check sampling) the flow rate of the sampling method does not move enough air to reliably pick up surface contamination unless the mold genera/species happens to be at a particularly high state of active sporulation. The tool remains in the professional's arsenal, to be used with discretion.

Shortcomings of vacuuming building cavities through a tube but our testing strongly suggests that this method is very unreliable for characterizing wall contents. We do not believe that enough air movement is created in the wall cavity (sucking any lpm flow through a small diameter tube) to reliably collect what could be a severe mold reservoir that happens not to be right next to the probe. Further if the cavity is insulated there will be virtually no air or particle movement except from very close to the probe.

What should we make of wall vacuum test results done without other inspection and testing?

Wall cavity vacuuming (C) Daniel FriedmanWall cavity vacuum tests, depending on how and when they are performed, can be like searching for a needle in a haystack while looking through a straw: if you find evidence of a problem you're lucky. But if you don't find evidence, when using a very limited-scope method, that doesn't mean that a problem is not there.

Our photo, left, is not showing the wall-check vacuum method that relies on vacuuming into a cassette through a hole in the wall. Instead, here we have pulled loose paneling and have inserted our spore trap cassette into the cavity.

This position combined with aggressive sampling by banging on the wall paneling with a flashlight is more likely to collect problem particles if they are present close to the point of vacuum cassette insertion into the wall. But even this approach is not a reliable characterization of mold risk in the building. And it is not looking into the wall cavity itself - rather we are looking at the two surfaces behind the paneling.

Wall vacuum test (C) Daniel FriedmanShort vacuum pump duration for microscopic examination (to avoid sample overload, e.g. 2-=3 minutes on an Air-O-Cell) does not move enough air to reliably find what may be in the wall cavity. An experiment done with Louis Relle in New Orleans LA demonstrated that wall-vac tests found less than 10% of large problem mold cavities that could be discovered by cutting drywall openings in a building.

Our photo (left) shows a short-duration but larger surface area or wall opening vacuuming system invented and tested by the author - it did not provide useful results.

Longer vacuum pump duration samples for viable sampling (2 hours into an MCE cassette for culturing) still may not move enough air to sample through a cavity, particularly if the cavity is insulated. Further, two-hour samples means that most-likely very few sample points were collected, making the inspection scope extremely limited and thus overall confidence in the accuracy of the picture of the building lower.

Consider that the popularly-marketed version of this wall cavity vacuuming approach to test for hidden mold contamination in buildings relies on culture of the sample. Did you know that only about 10% of molds grow in any culture at all? You're 90% uncertain of the accuracy of the test at the outset. One can't be sure that the mold that grew in the culture represents the dominant problem mold or whether it's just a low-occurrence (in the building) spore that liked the media (in the culture). We like cultures for further genera/species identification of samples but we are nervous about relying on them to tell me if the building has a problem or not.


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Technical Reviewers & References

Related Topics, found near the top of this page suggest articles closely related to this one.

  • Kansas State University, department of plant pathology, extension plant pathology web page on wheat rust fungus: see http://www.oznet.ksu.edu/path-ext/factSheets/Wheat/Wheat%20Leaf%20Rust.asp
  • "A Brief Guide to Mold, Moisture, and Your Home", U.S. Environmental Protection Agency US EPA - includes basic advice for building owners, occupants, and mold cleanup operations. See http://www.epa.gov/mold/moldguide.htm
  • US EPA - Mold Remediation in Schools and Commercial Building [Copy on file at /sickhouse/EPA_Mold_Remediation_in_Schools.pdf ] - US EPA
  • US EPA - Una Breva Guia a Moho - Hongo [Copy on file as /sickhouse/EPA_Moho_Guia_sp.pdf - en Espanol
  • "IgG Food Allergy Testing by ELISA/EIA, What do they really tell us?" Sheryl B. Miller, MT (ASCP), PhD, Clinical Laboratory Director, Bastyr University Natural Health Clinic - ELISA testing accuracy: Here is an example of Miller's critique of ELISA http://www.betterhealthusa.com/public/282.cfm - Townsend Letter for Doctors and Patients
    The critique included in that article raises compelling questions about IgG testing assays, which prompts our interest in actually screening for the presence of high levels of particles that could carry allergens - dog dander or cat dander in the case at hand.
    http://www.tldp.com/issue/174/IgG%20Food%20Allergy.html contains similar criticism in another venue but interestingly by the same author, Sheryl Miller. Sheryl Miller, MT (ASCP), PhD, is an Immunologist and Associate Professor of Basic and Medical Sciences at Bastyr University in Bothell, Washington. She is also the Laboratory Director of the Bastyr Natural Health Clinic Laboratory.
  • Allergens: Testing for the level of exposure to animal allergens is discussed at http://www.animalhealthchannel.com/animalallergy/diagnosis.shtml (lab animal exposure study is interesting because it involves a higher exposure level in some cases
  • Allergens: WebMD discusses allergy tests for humans at webmd.com/allergies/allergy-tests
  • Atlas of Clinical Fungi, 2nd Ed., GS deHoog, J Guarro, J Gene, & MJ Figueras, Centraalbureau voor Schimmelcultures, Universitat Rovira I Virgili, 2000, ISBN 90-70351-43-9 (you can buy this book at Amazon) - The Atlas of Clinical Fungi is also available on CD ROM
  • "A Brief Guide to Mold, Moisture, and Your Home", U.S. Environmental Protection Agency US EPA - includes basic advice for building owners, occupants, and mold cleanup operations. See http://www.epa.gov/mold/moldguide.htm
  • "Disease Prevention Program for Certain Vegetable Crops," David B. Langston, Jr., Extension Plant Pathologist - Vegetables, University of Georgia (PDF document) original source: www.reeis.usda.gov/web/crisprojectpages/209797.html
  • "Disease Prevention in Home Vegetable Gardens," Patricia Donald, Department of Plant Microbiology and Pathology, Lewis Jett
    Department of Horticulture, University of Missouri Extension - extension.missouri.edu/publications/DisplayPub.aspx?P=G6202
  • "Management of Powdery Mildew, Leveillula taurica, in Greenhouse Peppers," Ministry of Agriculture and Lands, British Columbia - Original source: www.agf.gov.bc.ca/cropprot/peppermildew.htm
  • Environmental Health & Investigation Bibliography - our own technical library on indoor air quality inspection, testing, laboratory procedures, forensic microscopy, etc.
  • Fiberglass: Mold in Fiberglass Insulation© 2005 comments about a field study in process, & more about health hazards from fiberglass insulation - DJF
  • Fifth Kingdom, Bryce Kendrick, ISBN13: 9781585100224, is available from the InspectAPedia online bookstore - we recommend the CD-ROM version of this book. This 3rd/edition is a compact but comprehensive encyclopedia of all things mycological. Every aspect of the fungi, from aflatoxin to zppspores, with an accessible blend of verve and wit. The 24 chapters are filled with up-to-date information of classification, yeast, lichens, spore dispersal, allergies, ecology, genetics, plant pathology, predatory fungi, biological control, mutualistic symbioses with animals and plants, fungi as food, food spoilage and mycotoxins.
  • Fungi, Identifying Filamentous, A Clinical Laboratory Handbook, Guy St-Germain, Richard Summerbell, Star Publishing, 1996, ISBN 0-89863-177-7 (English) (buy at Amazon)
  • US EPA: Mold Remediation in Schools and Commercial Building [Copy on file at /sickhouse/EPA_Mold_Remediation_in_Schools.pdf ] - US EPA
  • Mycology, Fundamentals of Diagnostic, Fran Fisher, Norma B. Cook, W.B. Saunders Co. 1998, ISBN 0-7216-5006-6 (buy this book at Amazon)
  • US EPA: Una Breva Guia a Moho - Hongo [Copy on file as /sickhouse/EPA_Moho_Guia_sp.pdf - en Espanol
  • Mark Cramer Inspection Services Mark Cramer, Tampa Florida, Mr. Cramer is a past president of ASHI, the American Society of Home Inspectors and is a Florida home inspector and home inspection educator. (727) 595-4211 mark@BestTampaInspector.com

Books & Articles on Building & Environmental Inspection, Testing, Diagnosis, & Repair

  • Our recommended books about building & mechanical systems design, inspection, problem diagnosis, and repair, and about indoor environment and IAQ testing, diagnosis, and cleanup are at the InspectAPedia Bookstore. Also see our Book Reviews - InspectAPedia.
  • Home Reference Book - Carson DunlopThe Home Reference Book - the Encyclopedia of Homes, Carson Dunlop & Associates, Toronto, Ontario, 25th Ed., 2012, is a bound volume of more than 450 illustrated pages that assist home inspectors and home owners in the inspection and detection of problems on buildings. The text is intended as a reference guide to help building owners operate and maintain their home effectively. Field inspection worksheets are included at the back of the volume. Special Offer: For a 10% discount on any number of copies of the Home Reference Book purchased as a single order. Enter INSPECTAHRB in the order payment page "Promo/Redemption" space. InspectAPedia.com editor Daniel Friedman is a contributing author.

    Or choose the The Home Reference eBook for PCs, Macs, Kindle, iPad, iPhone, or Android Smart Phones. Special Offer: For a 5% discount on any number of copies of the Home Reference eBook purchased as a single order. Enter INSPECTAEHRB in the order payment page "Promo/Redemption" space.

  • GO TO Carson Dunlop's Home Study Course Information - How to Become a Home Inspector: Carson Dunlop's nationally recognized Home Study Course, selected by ASHI the American Society of Home Inspectors and other professionals and associations. This website author is a contributor to this course.
  • GO TO Carson Dunlop's Home Study Course Information - How to Become a Home Inspector: Carson Dunlop's nationally recognized Home Study Course, selected by ASHI the American Society of Home Inspectors and other professionals and associations. This website author is a contributor to this course.
  • GO TO Carson Dunlop's Home Study Course Information - How to Become a Home Inspector: Carson Dunlop's nationally recognized Home Study Course, selected by ASHI the American Society of Home Inspectors and other professionals and associations. This website author is a contributor to this course.
    Building inspection education & report writing systems from Carson, Dunlop & Associates Ltd
  • Environmental Health & Investigation Bibliography - our technical library on indoor air quality inspection, testing, laboratory procedures, forensic microscopy, etc.
  • ...

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