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 Mobile ViewENVIRONMENTAL HAZARDS - INSPECT, TEST, REMEDY MOLD: A COMPLETE GUIDE to TEST CLEAN PREVENT ACCEPTABLE MOLD LEVEL ACCURACY OF VARIOUS MOLD TEST METHODS ACTION GUIDE - WHAT TO DO ABOUT INDOOR MOLD ACTIVITY of MOLD in buildings AGE of MOLD - Old is the Mold? AIR TEST FOR MOLD: ACCURACY AIR TEST SAMPLING CASSETTE STUDY AIRBORNE MOLD SPORE COUNT ACCURACY AIRBORNE PARTICLE ANALYSIS METHODS ALLERGEN TESTS for buildings ATTORNEYS and EXPERT WITNESSES ATTIC MOLD BASEMENT MOLD BASEMENT MOLD WATER IMPACT BATHROOM MOLD BIBLIOGAPHY for ENVIRONMENTAL HEALTH, MOLD, IAQ BIOLOGICAL POLLUTANTS BLACK MOLD, HARMLESS COSMETIC BLACK MOLD, TOXIC & ALLERGENIC BLEACHING MOLD, Advice about BOOK MOLD, Moldy Book Cleaning BOOKSTORE - ENVIRONMENTAL BROWN HAIRY BATHROOM MOLD CACTUS FUNGI / MOLD CAR MOLD CONTAMINATION CARPET DUST IDENTIFICATION CARPETING & INDOOR AIR QUALITY CHAIN OF CUSTODY - TEST SAMPLE CLEARANCE INSPECTIONS - MOLD CLEANUP CRAWL SPACES DIRECTORY of MOLD / ENVIRONMENTAL EXPERTS DIRT FLOOR MOLD CONTAMINATION Disinfectants Disinfecting Buildings with Bleach DRYWALL MOLD DUCT SYSTEM & DUCT DEFECTS DUST ANALYSIS for FIBERGLASS DUST CONTAMINATION FROM HVAC? DUST SAMPLING PROCEDURE EFFLORESCENCE, Salts & White / Brown Deposits EMERGENCY RESPONSE, IAQ, GAS, MOLD FEAR of MOLD - MYCOPHOBIA Fiberboard Insulation Sheathing Mold FIBERGLASS INSULATION MOLD FIBERGLASS PARTICLE CONTAMINATION TEST FIELD INVESTIGATION SERVICE FIND MOLD, ESSENTIAL STEPS FIND MOLD in BUILDINGS, HOW TO FIRE DAMAGE vs MOLD DAMAGE FLOOD DAMAGE ASSESSMENT, SAFETY & CLEANUP FLOODS IN BUILDINGS-mold FLOORING MATERIALS, Age, Types FUNGICIDAL SPRAY & SEALANT USE GUIDE GAS EXPOSURE EFFECTS, TOXIC GAS DETECTION & MEASUREMENT HOUSE DUST ANALYSIS HOUSEWRAP AIR & VAPOR BARRIERS HIDDEN MOLD, HOW TO FIND HUMIDITY CONTROL & TARGETS INDOORS INDOOR AIR HAZARDS TABLE INDOOR AIR QUALITY & HOUSE TIGHTNESS INDOOR AIR QUALITY IMPROVEMENT GUIDE INSULATION INSPECTION & IMPROVEMENT INSULATION MOLD ITCHY FABRICS LABORATORY SERVICES LAB PROCEDURES MICROSCOPE TECHNIQUES MICROSCOPE DIGITAL PHOTOGRAPHY Digital Photography & the Microscope Introduction to Digital Photography and Microscopy Some Factors Involved in Obtaining and Using Good Photomicrographs The Digital Image Formation Chain Selection of Digital Cameras Camera mounts Camera Settings Automatic Settings Image print size Manual setting mode Focusing tips for Digital Cameras Take some test photos Getting your photos from camera to the computer Simple method - flash card Manufacturer's cables Video bus Building and Organizing a Digital Photo Library on a computer Photo library management Photo Library design First Pass Screening Saccardo Method Image Library Backup Suggestions References for Digital Photography MICROSCOPE SLIDE PREPARATION Slide Preparation Procedure Slide Stain & Media Recipes Permanent Slide Mounts Gelvatol Meltmount Mowiol The Stains File MICROSCOPE SLIDE PREP - PERMANENT MOUNTS Required materials To use meltmount slides MICROSCOPE TECHNIQUES for the LAB MEDIA BLASTING for MOLD REMOVAL METHANE GAS SOURCES MICROSCOPE DIGITAL PHOTOGRAPHY MILDEW in BUILDINGS ? MILDEW ERRORS - MOLD PHOTOS MILDEW REMOVAL & PREVENTION MOISTURE CONTROL in BUILDINGS MOLD by MICROSCOPE MOLD: A COMPLETE GUIDE TO MOLD MOLD ACTION GUIDE - WHAT TO DO ABOUT MOLD MOLD APPEARANCE - WHAT MOLD LOOKS LIKE MOLD CLEANERS - WHAT TO USE MOLD CLEANUP GUIDE- HOW TO GET RID OF MOLD MOLD CONSULTANTS/INSPECTORS MOLD CULTURE TEST KIT VALIDITY MOLD DETECTION & INSPECTION GUIDE MOLD or INDOOR AIR EMERGENCY RESPONSE MOLD EXPERT, WHEN TO HIRE MOLD FAQ's MOLD ODORS, MUSTY SMELLS MOLD PREVENTION GUIDE MOLD RELATED ILLNESS GUIDE MOLD SAFETY WARNINGS MOLD SPRAYS, SEALANTS, PAINTS MOLD STANDARDS MOLD TEST KITS for DIY MOLD TESTS MOLD TESTING METHOD VALIDITY MSDS Material Safety Data Sheets MSDS Material Safety Data Sheets MVOCs & MOLDY MUSTY ODORS MYCOPHOBIA, STAINS MISTAKEN for MOLD MYCOTOXIN EFFECTS of MOLD EXPOSURE ODORS GASES SMELLS, DIAGNOSIS & CURE OZONE for MOLD OR ODORS PAINT ANALYSIS, DIAGNOSTIC USES PAINT & STAIN GUIDE, EXTERIOR PAINT FALURE, DIAGNOSIS, CURE, PREVENTION PAINT FAILURE CHECKLIST PAINTING MISTAKES PAINT FAILURE DICTIONARY PAINT FAILURE ANALYSIS LAB PHOTOS Paint Failure Case Photographs-SITE Paint Failure Case Photographs-LAB PAINT LAB SAMPLE PREPARATION PAINT SURFACE PREPARATION PARTICLE SIZES & IAQ Particulates & Allergens Indoors Pesticide Exposure Hazards PET ALLERGEN REMEDIES POLLEN Photographs RENTERS & TENANTS GUIDE TO MOLD ROBIGUS & Wheat Rust Fungus ROT, TIMBER FRAME SLIDE PREPARATION, MICROSCOPE SMELL PATCH TEST to Track Down Odors SOUND CONTROL in BUILDINGS STAIN & BIODETERIORATION AGENT CATALOG STAINS on BUILDINGS - QUICK GUIDE STAIN DIAGNOSIS on BUILDING EXTERIORS STAIN DIAGNOSIS on BUILDING INTERIORS STAINS on INDOOR SURFACES: PHOTO GUIDE STAINS & Thermal Tracking TECHNICAL & LAB PROCEDURES TRAPPED MOLD BETWEEN WOOD SURFACES VAPOR BARRIERS & CONDENSATION in BUILDINGS VENTILATION in buildings VINYL Siding or PLASTIC Window ODORS in buildings VINYL CHLORIDE HEALTH INFO WATER BARRIERS, EXTERIOR BUILDING WATER ENTRY in buildings World Trade Center Collapse Dust Photos More Information |
Digital microphotography guide: The purpose of this paper is to help microscopists and other photographers take high quality digital photographs, store them for easy editing and retrieval, and use digital microphotographs as an information or forensic database. We discuss using modern digital cameras and transmitted-light microscopes together. InspectAPedia tolerates no conflicts of interest. We have no relationship with advertisers nor with topics or services discussed at this website.© Copyright 2012 InspectAPedia.com, All Rights Reserved. Information Accuracy & Bias Pledge is at below-left. Use page top links to major topics or use links at the left of each page to navigate within topics and documents at this website. Green links show where you are in a document series or at this website. Introduction to Digital Photography and Microscopy PAAA 2005 Symposium, University of Tulsa, Tulsa Oklahoma -
June 2-5, 2005 - updated 02/23/2009 This article discusses the selection of cameras that adapt well to microscope eyepieces or trinocular heads, and on camera settings and procedures to obtain best quality photographs. We also discuss image resolution and size settings and make recommendations. Cameras used as examples in this paper include the Nikon Coolpix series 990, 995, and 4500. The techniques discussed in this paper work well with digital cameras and any type of microscope, low power stereo zoom to high power forensic polarized light microscopes.] Before specifying a camera choice and recommending camera settings let's review the purposes of digital microphotography of particular interest to aerobiologists and other microscopists:
Some Factors Involved in Obtaining and Using Good PhotomicrographsStart with a well prepared slide, choosing a mountant with good optics for the particles being examined. Fungal spores or pollen grains may benefit from hydration but not excessive hydration. Heavy use of colored stains risks obscures natural colors and features needed for identification, though we may succumb and use fuchsin, lacto phenol, or even India ink for occasional development of certain features. After a well prepared and clean slide, be sure the microscope is properly set up for Koehler illumination, and be sure the objective is clean. Select a camera which has a very good lens - among the current crop of 3+megapixel cameras, this is perhaps more important than a higher number of pixels. Lens resolution in macro mode and with the camera focused at infinity are both important. Post processing of images may improve the image color balance, sharpness, or contrast, but no amount of processing can produce accurate image data if that information was not captured in the first place. Even excellent photomicrographs are not useful if they cannot be located later for reference purposes. A good photographic data base system is important. The Digital Image Formation Chain determines image quality in digital photos through the microscope
What this list means is that the quality of the end result of photomicrography is limited by the weakest link in the image-formation chain. For example, no amount of camera pixels will provide a sharp image of an object which is not resolved sharply in the microscope nor will a high-pixel camera produce a sharp image if the camera's lens is of limited ability. Beware: not all high pixel cameras have equally sharp lenses, so the "resolution" in megapixels can be misleading. High megapixels defines how many data points of image are being recorded. But if the lens and other steps in the image formation chain are not producing a sharp image, high megapixels means you're recording a lot of fuzzy data. With these preliminaries, let's look at choosing a camera, selecting camera settings, using mounts, etc. Questions & Answers regarding this articleQuestions & answers about digital microphotography: how to take succcessful, sharp digital photographs through the microscope Ask a Question or Search InspectAPediaHTML Comment Box is loading comments...
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Use links just below or at the left of each page to navigate this document or to view other topics at this website. Green links show where you are in our document or website. Digital Photography & the Microscope References for Digital Photography Through the Microscope - Micro PhotographyA Short Course in Nikon Coolpix 990 Photography, Dennis P. Curtain, http://www.shortcourses.com - RECOMMENDED Resolution of Digital Photomicrographs from Scanned Film, Theodore M. Clark, Microscopy Today, Feb/Mar 2001 http://www.couger.com/microscope/Ted-Clarke/papers/FilmScanner/ Light, Michael I. Sobel, University of Chicago Press, 1987, ISBN 0-226-76751-5 ThumbsPlus® image software, provides highly functional image database including basic editing, cropping, enlarging, enhancement of photos, image organization, keywords and comments attached to each image, and search capability - HIGHLY RECOMMENDED for PC platforms. Typically less than $100. Network support available. ® image editing software, provides sophisticated image editing, available for PC and Macintosh computer platforms. Not recommended for reference library use - this is overkill for image editing and lacks good database functions. Typically more than $400. iPhoto® image software, provides easy user interface and limited function to organize and print photos for MAC computer platforms - not recommended, limited function, very wasteful of disk space, lacks good database functions. Free included with new Apple computers or operating systems. Photo Explosion® image software - free from Microsoft with other OS purchases. Untried. Picture Easy software from Kodak - free with some Kodak cameras, simple organizing and photo manipulation. Nikon View® software - free from Nikon with purchase of Coolpix cameras, easy image import and simple image manipulation and printing. Great for printing contact sheets of photos (which themselves are pretty useless). [1] Thumbs Plus image data base software from Cerious software, www.cerious.com is a top choice low-cost option), and will be searched-through for reference purposes Books & Articles on Building & Environmental Inspection, Testing, Diagnosis, & Repair
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